Acid-sensing ion channels contribute to the effect of acidosis on the function of dendritic cells.

Abstract	As an H(+)-gated subgroup of the degenerin/epithelial Na(+) channel family, acid-sensing ion channels (ASICs) were reported to be involved in various physiological and pathological processes in neurons. However, little is known about the role of ASICs in the function of dendritic cells (DCs). In this study, we investigated the expression of ASICs in mouse bone marrow-derived DCs and their possible role in the function of DCs. We found that ASIC1, ASIC2, and ASIC3 are expressed in DCs at the mRNA and protein levels, and extracellular acid can evoke ASIC-like currents in DCs. We also demonstrated that acidosis upregulated the expression of CD11c, MHC class II, CD80, and CD86 and enhanced the Ag-presenting ability of DCs via ASICs. Moreover, the effect of acidosis on DCs can be abolished by the nonsteroidal anti-inflammatory drugs ibuprofen and diclofenac. These results suggest that ASICs are involved in the acidosis-mediated effect on DC function.
Authors	Jing Tong, Wen-Ning Wu, Xiaoling Kong, Peng-Fei Wu, Li Tian, Wenjiao Du, Min Fang, Fang Zheng, Jian-Guo Chen, Zheng Tan, Feili Gong
Journal	Journal of immunology (Baltimore, Md. : 1950) (J Immunol) Vol. 186 Issue 6 Pg. 3686-92 (Mar 15 2011) ISSN: 1550-6606 [Electronic] United States
PMID	21321108 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	ASIC1 protein, mouse ASIC2 protein, mouse ASIC3 protein, rat Acid Sensing Ion Channels Asic1 protein, rat Nerve Tissue Proteins Sodium Channels
Topics	Acid Sensing Ion Channels Acidosis (immunology, metabolism, pathology) Animals Animals, Newborn Cell Differentiation (immunology) Cells, Cultured Dendritic Cells (cytology, immunology, metabolism) Extracellular Space (immunology, metabolism) Female Hydrogen-Ion Concentration Mice Mice, Inbred BALB C Mice, Inbred C57BL Nerve Tissue Proteins (biosynthesis, physiology) Rats Rats, Sprague-Dawley Sodium Channels (biosynthesis, physiology)

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