The differentiation of the newborn nerve cells in oculomotor nuclear after oculomotor nerve injury.

Abstract

Oculomotor nerve injury is a common complication of cranial trauma and craniotomy. For a long time, it has been generally considered that the oculomotor nerve is unable to regenerate and recover functionally after injury. With the development of neuroradiology, microsurgery and neurohistology, it has been reported that the injured oculomotor nerve could be repaired by operation. However, the mechanisms of neural regeneration of the injured oculomotor nerve remain obscure. Therefore, by investigating the differentiation of the newborn nerve cells in oculomotor nuclear after oculomotor nerve injury, the mechanisms of the neural regeneration of the injured oculomotor nerve was studied in the present paper. After animal model establishment, we found that the function of the injured oculomotor nerve could recover at some degree without treatment, at fourth week after the nerve injury. This result confirms that the injured oculomotor nerve per se has the potential to regenerate and repair. At the present study, by BredU stain, BrdU labeling cells were observed in oculomotor nuclear at the fourth week post-operatively. It indicated that the oculomotor nuclear per se has the ability of generating the cells, which will regenerate and differentiate after the nerve injury, without stimulation by exogenous agents. Immunofluorescence double staining was used in this study to show the differentiation of the newborn cells in oculomotor nuclear after oculomotor nerve injury. It is found that they could differentiate into neural progenitor cells, neuronal cells and neuroglial cells. It is suggested that the different differentiation of cells may play a role in the nerve regeneration procedure.

• Authors: Min Yang, Ningxi Zhu, Youqiang Meng, Xuhui Wang, Jun Zhong, Liang Wan, Wenchuan Zhang, Massimiliano Visocchi, Shugan Zhu, Shiting Li
• Journal: Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology (Neurol Sci) Vol. 32 Issue 2 Pg. 281-6 (Apr 2011) ISSN: 1590-3478 [Electronic] Italy
• PMID: 21301911 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Topics

• Animals
• Cell Differentiation (physiology)
• Dogs
• Fluorescent Antibody Technique
• Immunohistochemistry
• Mesencephalon (cytology)
• Microscopy, Confocal
• Nerve Crush
• Nerve Regeneration (physiology)
• Neural Stem Cells (cytology)
• Neurons (cytology)
• Oculomotor Nerve (cytology)
• Oculomotor Nerve Injuries