Aerosol vaccination induces robust protective immunity to homologous and heterologous influenza infection in mice.

Abstract

Live-attenuated influenza vaccine (LAIV) delivered by large droplet intranasal spray is efficacious against infection. However, many of the large droplets are trapped in the external nares and do not reach the target nasal airway tissues. Smaller droplets might provide better distribution yielding similar protection with lower doses. We evaluated 20 and 30 μm aerosol delivery of influenza virus in mice. A 15s aerosol exposure optimally protected against homologous and heterologous influenza infection and induced a robust immune response. These results demonstrate the feasibility of nasal vaccination using aerosolized particles, providing a strategy to improve vaccine efficacy and delivery.

• Authors: Jennifer Humberd Smith, Paula Brooks, Scott Johnson, S Mark Tompkins, Koren M Custer, Debra L Haas, Raydel Mair, Mark Papania, Ralph A Tripp
• Journal: Vaccine (Vaccine) Vol. 29 Issue 14 Pg. 2568-75 (Mar 21 2011) ISSN: 1873-2518 [Electronic] Netherlands
• PMID: 21300100 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, P.H.S.)
• Copyright: Copyright © 2011 Elsevier Ltd. All rights reserved.

Chemical References

• Aerosols
• Antibodies, Viral
• Influenza Vaccines

Topics

• Administration, Intranasal
• Aerosols (administration & dosage)
• Animals
• Antibodies, Viral (blood)
• Enzyme-Linked Immunosorbent Assay
• Female
• Hemagglutination Inhibition Tests
• Influenza Vaccines (administration & dosage, immunology)
• Mice
• Mice, Inbred BALB C
• Orthomyxoviridae Infections (prevention & control)
• Vaccination (methods)