Ultrastructural immunocytochemistry in glial tumors.

Abstract

We have studied the ultrastructural distribution of GFAP, vimentin, FVIII/RAg and collagen IV in biopsies of human gliomas with various degree of anaplasia. Pre-embedding (vibratome and cryoultrathin sections) and post-embedding (LR White sections) techniques were tested. The immune reaction was demonstrated by peroxidase-DAB and gold labeling. In astrocytes, GFAP and VIM were localized in the same filament bundles; GFAP was always more expressed than vimentin, regardless of the malignancy grade. FVIII was localized in endoplasmic reticulum and in Weibel-Palade bodies. The influence of technical procedures on the optimal demonstration of each antigen is stressed.

• Authors: A Migheli, C Mocellini
• Journal: Journal of neurosurgical sciences (J Neurosurg Sci) 1990 Jul-Dec Vol. 34 Issue 3-4 Pg. 219-22 ISSN: 0390-5616 [Print] Italy
• PMID: 2129126 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Glial Fibrillary Acidic Protein
• Vimentin
• Factor VIII
• Collagen

Topics

• Collagen (analysis)
• Factor VIII (analysis)
• Glial Fibrillary Acidic Protein (analysis)
• Glioma (chemistry, ultrastructure)
• Humans
• Immunohistochemistry
• Vimentin (analysis)