Abstract |
OX40 protein is highly expressed on activated CD4-positive T cells that are susceptible for human immunodeficiency virus type 1 (HIV-1) infection. To target and kill HIV-1-infected OX40(+) T cells, we used a recombinant vesicular stomatitis virus (rVSV) lacking its envelope glycoprotein (ΔG) and instead expressing HIV-1 receptors CD4/CXCR4 and OX40 ligand (OX40L). Expression of OX40L as well as HIV-1 receptors on the VSV particles led to specific infection of OX40(+) T cells, including primary cells, either acutely or chronically infected with X4 HIV-1. Consequently, the rVSV rapidly eliminated these infected cells and caused a marked reduction of HIV-1 viral load in culture. Inclusion of the OX40L gene in the VSV recombinant led to significantly better infection and HIV-1 elimination compared with an rVSVΔG expressing only HIV-1 receptors. A novel rVSVΔG encoding both HIV-1 receptors and OX40L has a potentially greater therapeutic value than an rVSVΔG expressing only HIV-1 receptors.
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Authors | Chikayuki Tsuruno, Kazu Okuma, Yoshiaki Takahashi, Reiko Tanaka, Yuetsu Tanaka, Youichi Takahama, Yukio Hamaguchi, Isao Hamaguchi, Kazunari Yamaguchi |
Journal | Human immunology
(Hum Immunol)
Vol. 72
Issue 4
Pg. 295-304
(Apr 2011)
ISSN: 1879-1166 [Electronic] United States |
PMID | 21262309
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2011 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved. |
Chemical References |
- CD4 Antigens
- OX40 Ligand
- Receptors, CXCR4
- Receptors, OX40
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Topics |
- Animals
- CD4 Antigens
(genetics, immunology, metabolism)
- CD4-Positive T-Lymphocytes
(immunology, virology)
- Cell Line
- Cell Line, Tumor
- Cricetinae
- Gene Order
- Genetic Vectors
(genetics, isolation & purification, metabolism)
- Giant Cells
(metabolism, pathology)
- HEK293 Cells
- HIV Infections
(immunology, virology)
- HIV-1
(immunology, metabolism)
- Humans
- OX40 Ligand
(genetics, immunology)
- Receptors, CXCR4
(genetics, immunology, metabolism)
- Receptors, OX40
(immunology)
- Vesiculovirus
(genetics, isolation & purification, metabolism)
- Viral Load
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