Cloning and characterization of the aroA gene from Mycobacterium tuberculosis.

Abstract

The aroA gene from Mycobacterium tuberculosis has been cloned by complementation of an aroA mutant of Escherichia coli after lysogenization with a recombinant DNA library in the lambda gt11 vector. Detailed characterization of the M. tuberculosis aroA gene by nucleotide sequencing and by immunochemical analysis of the expressed product indicates that it encodes a 5-enolpyruvylshikimate-3-phosphate synthase that is structurally related to analogous enzymes from other bacterial, fungal, and plant sources. The potential use of the cloned gene in construction of genetically defined mutant strains of M. tuberculosis by gene replacement is proposed as a novel approach to the rational attenuation of mycobacterial pathogens and the possible development of new antimycobacterial vaccines.

• Authors: T Garbe, C Jones, I Charles, G Dougan, D Young
• Journal: Journal of bacteriology (J Bacteriol) Vol. 172 Issue 12 Pg. 6774-82 (Dec 1990) ISSN: 0021-9193 [Print] United States
• PMID: 2123856 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA, Bacterial
• Transferases
• Alkyl and Aryl Transferases
• 3-Phosphoshikimate 1-Carboxyvinyltransferase

Topics

• 3-Phosphoshikimate 1-Carboxyvinyltransferase
• Alkyl and Aryl Transferases
• Amino Acid Sequence
• Base Sequence
• Blotting, Southern
• Blotting, Western
• Cloning, Molecular
• DNA, Bacterial (genetics)
• Genes, Bacterial
• Genetic Complementation Test
• Molecular Sequence Data
• Molecular Weight
• Mycobacterium tuberculosis (genetics)
• Restriction Mapping
• Transferases (genetics)