Metabonomic investigation of single and multiple strain Trypanosoma brucei brucei infections.

Abstract

Although co-infections are common and can have important epidemiologic and evolutionary consequences, studies exploring biochemical effects of multiple-strain infections remain scarce. We studied metabolic responses of NMRI mice to Trypanosoma brucei brucei single (STIB777AE-Green1 or STIB246BA-Red1) and co-infections using a (1)H nuclear magnetic resonance (NMR) spectroscopy-based metabolic profiling strategy. All T. b. brucei infections caused an alteration in urinary biochemical composition by day 4 postinfection, characterized by increased concentrations of 2-oxoisocaproate, D-3-hydroxybutyrate, lactate, 4-hydroxyphenylacetate, phenylpyruvate, and 4-hydroxyphenylpyruvate, and decreased levels of hippurate. Although there were no marked differences in metabolic signatures observed in the mouse infected with a single or dual strain of T. b. brucei, there was a slower metabolic response in mice infected with T. b. brucei green strain compared with mice infected with either the red strain or both strains concurrently. Pyruvate, phenylpyruvate, and hippurate were correlated with parasitemia, which might be useful in monitoring responses to therapeutic interventions.

• Authors: Jia V Li, Jasmina Saric, Yulan Wang, Jürg Utzinger, Elaine Holmes, Oliver Balmer
• Journal: The American journal of tropical medicine and hygiene (Am J Trop Med Hyg) Vol. 84 Issue 1 Pg. 91-8 (Jan 2011) ISSN: 1476-1645 [Electronic] United States
• PMID: 21212208 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Water

Topics

• Animals
• Feces (chemistry)
• Female
• Humans
• Metabolome (physiology)
• Mice
• Parasitemia
• Trypanosoma brucei brucei (classification)
• Trypanosomiasis, African (metabolism, parasitology, urine)
• Water (chemistry)