Amylase and lipase detection in hemorrhaged animals treated with HBOC-201.

Abstract

HBOC-201 may alter lipase and amylase detection on chemistry analyzers using optical methods and affect pancreatic function after trauma. Amylase and lipase measurements were correlated against HBOC-201 to evaluate interference on samples spiked with 0-6g/dL HBOC-201. The detection threshold was 2.5g/dL or none when measured, respectively, on Vitros 250 or Advia 1650 instruments. Amylase and lipase from blood samples collected from 55% EBV hemorrhaged Yucatan min-pigs showed peaks around 24-48 hours. Amylase increase was not significant between treatments but lipase was higher in HBOC-201-treated animals. Animals particularly affected by the injury had elevated enzymes after hemorrhagic shock, without significant clinical consequences.

• Authors: Françoise Arnaud, Richard McCarron, Daniel Freilich
• Journal: Artificial cells, blood substitutes, and immobilization biotechnology (Artif Cells Blood Substit Immobil Biotechnol) Vol. 39 Issue 3 Pg. 155-61 (Jun 2011) ISSN: 1532-4184 [Electronic] England
• PMID: 21171938 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Blood Substitutes
• Hemoglobins
• HBOC 201
• Lipase
• Amylases

Topics

• Amylases (blood)
• Animals
• Blood Substitutes (administration & dosage)
• Hemoglobins (administration & dosage)
• Lipase (blood)
• Optical Phenomena
• Pancreas (injuries, physiopathology)
• Reference Values
• Shock, Hemorrhagic (blood, diagnosis, drug therapy, enzymology, etiology)
• Swine
• Swine, Miniature
• Wounds and Injuries (complications, diagnosis, drug therapy)