[Antiproliferation of cardamonin associated with mRNA expression of mTOR, Raptor and Rictor].

Abstract

OBJECTIVE:

To investigate the antiproliferation effect of cardamonin (CAR) and its possible mechanisms on human umbilical artery smooth muscle cells (HUASMCs) cultured in the mimicking insulin resistance (IR) medium.

METHOD:

Proliferation of HUASMCs was assayed by MTT method. The mRNA expression of mTOR, Raptor and Rictor was detected by a real-time PCR. The expression content was calculated by Livak method using internal control of beta-actin.

RESULT:

The proliferation of HUASMCs cultured in the mimicking IR medium was significantly increased. Both in normal and mimic IR culture medium, cells proliferation was inhibited by CAR (1 x 10(-5), 1 x 10(-4) mol x L(-1)). Pretreated with PD98059 and LY294002, cell proliferation induced by phosphatidic acid (PA) was inhibited, and the mRNA expression of mTOR, Raptor and Rictor was significantly decreased by CAR in the mimic IR medium.

CONCLUSION:

It is implicated that antiproliferation of CAR is involved in mRNA expression decrease of mTOR and its relative protein Raptor and Rictor.

• Authors: Wei Zheng, Daohua Shi, Xiangfu Ji, Ying Han, Qin Liao
• Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (Zhongguo Zhong Yao Za Zhi) Vol. 35 Issue 17 Pg. 2318-23 (Sep 2010) ISSN: 1001-5302 [Print] China
• PMID: 21137347 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Adaptor Proteins, Signal Transducing
• Carrier Proteins
• Chalcones
• Growth Inhibitors
• RICTOR protein, human
• RNA, Messenger
• RPTOR protein, human
• Rapamycin-Insensitive Companion of mTOR Protein
• Regulatory-Associated Protein of mTOR
• MTOR protein, human
• TOR Serine-Threonine Kinases
• cardamonin

Topics

• Adaptor Proteins, Signal Transducing (genetics, metabolism)
• Carrier Proteins (genetics, metabolism)
• Cell Proliferation (drug effects)
• Cells, Cultured
• Chalcones (pharmacology)
• Gene Expression Regulation (drug effects)
• Growth Inhibitors (pharmacology)
• Humans
• Myocytes, Smooth Muscle (cytology, drug effects, metabolism)
• RNA, Messenger (genetics, metabolism)
• Rapamycin-Insensitive Companion of mTOR Protein
• Regulatory-Associated Protein of mTOR
• TOR Serine-Threonine Kinases (genetics, metabolism)