Acute myocardial infarction is reflected in salivary matrix metalloproteinase-8 activation level.

Abstract

BACKGROUND:

The aim of this study is to compare salivary and serum biomarker levels and degrees of matrix metalloproteinase (MMP) activation between patients with acute myocardial infarction (AMI) and systemically healthy patients (non-AMI) with similar periodontal conditions.

METHODS:

A total of 92 patients (47 AMI and 28 non-AMI patients with gingivitis or periodontitis; and 17 systemically and periodontally healthy patients as a control group) were recruited. Clinical periodontal measurements were recorded; stimulated whole saliva and serum samples were collected. AMI patients were clinically examined within 3 to 4 days after admission to the coronary care unit. Saliva samples were analyzed for levels of MMP-8, MMP-7, and tissue inhibitor of matrix metalloproteinase (TIMP)-1. Serums were tested for MMP-8, MMP-9, TIMP-1, and TIMP-2 levels by immunofluorometric assay and enzyme-linked immunosorbent assay. Molecular forms and degree of activation of salivary MMP-8, MMP-9, and MMP-13 were analyzed by computer-scanned immunoblots.

RESULTS:

Total salivary MMP-8 assessed by immunofluorometric assay method and immunoblot densitometric units was higher in non-AMI than in AMI patients' saliva, but a significantly higher percentage of AMI patients' MMP-8 was activated polymorphonuclear leukocyte (PMN) type (P <0.001) regardless of periodontal diagnosis.Serum MMP-8, MMP-9, and TIMP-1 levels were significantly higher in AMI (for all markers and all comparisons,P <0.05). Characteristic for AMI was dominance of active PMN MMP-8 in saliva [corrected].

• Authors: Eralp Buduneli, Päivi Mäntylä, Gülnur Emingil, Taina Tervahartiala, Pirkko Pussinen, Nezihi Barış, Azem Akıllı, Gül Atilla, Timo Sorsa
• Journal: Journal of periodontology (J Periodontol) Vol. 82 Issue 5 Pg. 716-25 (May 2011) ISSN: 1943-3670 [Electronic] United States
• PMID: 21091346 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers
• Salivary Proteins and Peptides
• Tissue Inhibitor of Metalloproteinase-1
• Tissue Inhibitor of Metalloproteinase-2
• MMP13 protein, human
• Matrix Metalloproteinase 13
• MMP7 protein, human
• Matrix Metalloproteinase 7
• MMP8 protein, human
• Matrix Metalloproteinase 8
• Matrix Metalloproteinase 9

Topics

• Adult
• Aged
• Biomarkers (analysis, blood)
• Blotting, Western
• Chronic Periodontitis (complications, enzymology)
• Female
• Gingival Hemorrhage (classification)
• Gingivitis (complications, enzymology)
• Humans
• Male
• Matrix Metalloproteinase 13 (analysis)
• Matrix Metalloproteinase 7 (analysis)
• Matrix Metalloproteinase 8 (analysis, blood)
• Matrix Metalloproteinase 9 (analysis, blood)
• Middle Aged
• Myocardial Infarction (complications, enzymology)
• Periodontal Attachment Loss (classification)
• Periodontal Index
• Periodontal Pocket (classification)
• Periodontitis (complications, enzymology)
• Saliva (enzymology)
• Salivary Proteins and Peptides (analysis)
• Tissue Inhibitor of Metalloproteinase-1 (analysis, blood)
• Tissue Inhibitor of Metalloproteinase-2 (blood)