Biological activity of a gallic acid-gelatin conjugate.

Abstract

Goal of the present study was the characterization of the biological properties of a gelatin-gallic acid conjugate (Gel-GA) to evaluate its applicability in biomedicine and pharmacy. The macromolecular conjugate was synthesized by free radical grafting reaction between gelatin and gallic acid (GA) to form a covalent conjugate that was found to retain the antioxidant and enzymatic activities of free GA. In particular, the peroxynitrite scavenging power was found to be consistent with a IC(50) value of 2.17 ± 0.4 mg mL(-1). The enzymatic capacities of GA, which are regarded beneficial for cell functions, are partly retained in the Gel-GA conjugate. In particular, acetylcholinesterase inhibition (IC(50) of 7.1 ± 1.3 mg mL(-1)) implies the conjugate's usefulness in the chemoprevention of Alzheimer's disease, while the inhibition of α-amylase (IC(50) of 9.8 ± 1.1 mg mL(-1)) suggests that the conjugate can be a preferred alternative for inhibition of carbohydrate breakdown and control of glycemic index of food products. Finally, the anticancer activity of Gel-GA was proven in prostate carcinoma and renal cell carcinoma cell lines, confirming the potential of the proposed protein-polyphenol conjugate in medicine.

• Authors: Giuseppe Cirillo, Kai Kraemer, Susanne Fuessel, Francesco Puoci, Manuela Curcio, Umile Gianfranco Spizzirri, Ilaria Altimari, Francesca Iemma
• Journal: Biomacromolecules (Biomacromolecules) Vol. 11 Issue 12 Pg. 3309-15 (Dec 13 2010) ISSN: 1526-4602 [Electronic] United States
• PMID: 21058637 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Antioxidants
• Cholinesterase Inhibitors
• Gallic Acid
• Gelatin
• Acetylcholinesterase

Topics

• Acetylcholinesterase (drug effects)
• Alzheimer Disease (prevention & control)
• Antineoplastic Agents (chemical synthesis, pharmacology)
• Antioxidants (chemical synthesis)
• Carcinoma, Renal Cell (drug therapy, pathology)
• Cell Line, Tumor
• Cholinesterase Inhibitors (chemical synthesis)
• Female
• Gallic Acid (chemistry, therapeutic use)
• Gelatin (chemistry, therapeutic use)
• Humans
• Male
• Prostatic Neoplasms (drug therapy, pathology)