A propionyloxy derivative of 11-keto-β-boswellic acid induces apoptosis in HL-60 cells mediated through topoisomerase I & II inhibition.

Abstract

Boswellic acids have invariably been reported for their antiproliferative potential in various cell systems. In the present study the growth inhibitory effect of propionyloxy derivative of 11-keto-β-boswellic acid (PKBA; a semisynthetic analogue of 11-keto-β-boswellic acid) on HL-60 promyelocytic leukemia cells is being reported for the first time. In the preliminary studies, in vitro cytotoxicity of PKBA was investigated against eight human cancer cell lines viz., IMR-32, SF-295 (both neuroblastoma), PC-3 (prostate), Colo-205 (colon), MCF-7 (breast), OVCAR-5 (ovary), HL-60, Molt-4 (both leukemia) and their respective IC(50) values were found to be 5.95, 7.11, 15.2, 14.5, 15, 15.9, 8.7 & 9.5μg/ml, respectively. For determining the mechanism of cell death in HL-60 cells, PKBA was subjected to different mechanistic studies. DNA relaxation assay of PKBA revealed inhibition of both topoisomerases I & II. The fragmentation analysis of DNA revealed typical ladders indicating the cytotoxic effect to be mediated by induction of apoptosis. The morphologic studies of PKBA showed the presence of true apoptotic bodies. Apoptosis was confirmed further by flow-cytometric detection of sub-G(1) peaks and enhanced annexin-V-FITC binding of the cells. The activation of apoptotic cascade by PKBA in HL-60 cells was found to be associated with the loss of mitochondrial membrane potential, release of cytochrome c, activation of initiator and executioner caspases and cleavage of poly ADP ribose polymerase (PARP). In vivo studies of PKBA revealed anti-tumoral activity against both ascitic and solid murine tumor models. These studies thus demonstrate PKBA to induce apoptosis in HL-60 cells due to the inhibition of topoisomerases I and II.

• Authors: Gousia Chashoo, Shashank K Singh, Paraduman R Sharma, Dilip M Mondhe, Abid Hamid, Arpita Saxena, Samar S Andotra, Bhahwal A Shah, Naveed A Qazi, Subhash C Taneja, Ajit K Saxena
• Journal: Chemico-biological interactions (Chem Biol Interact) Vol. 189 Issue 1-2 Pg. 60-71 (Jan 15 2011) ISSN: 1872-7786 [Electronic] Ireland
• PMID: 21056033 (Publication Type: Journal Article)
• Copyright: Copyright © 2010 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• 11-keto-boswellic acid
• Propionates
• Topoisomerase Inhibitors
• Triterpenes
• DNA Topoisomerases, Type I
• DNA Topoisomerases, Type II

Topics

• Animals
• Apoptosis (drug effects)
• Cell Proliferation (drug effects)
• Comet Assay
• DNA Damage
• DNA Topoisomerases, Type I (metabolism)
• DNA Topoisomerases, Type II (metabolism)
• Flow Cytometry
• HL-60 Cells
• Humans
• Magnetic Resonance Spectroscopy
• Male
• Membrane Potential, Mitochondrial (drug effects)
• Mice
• Microscopy, Electron, Scanning
• Propionates (chemistry, pharmacology)
• Random Allocation
• Spectrometry, Mass, Electrospray Ionization
• Topoisomerase Inhibitors (pharmacology)
• Triterpenes (chemistry, pharmacology)