Evaluation of three commercially available Japanese encephalitis virus IgM enzyme-linked immunosorbent assays.

Abstract

We evaluated performance of three commercial Japanese encephalitis virus (JEV) IgM antibody capture enzyme-linked immunosorbent assay (MAC ELISA) kits with a panel of serological specimens collected during a surveillance project of acute encephalitis syndrome in India and acute meningitis and encephalitis syndrome in Bangladesh. The serum and cerebral spinal fluid specimens had been referred to the Centers for Disease Control and Prevention (CDC) for confirmatory testing. The CDC results and specimen classifications were considered the reference standard. All three commercial kits had high specificity (95-99.5%), but low sensitivities, ranging from 17-57%, with both serum and cerebrospinal fluid samples. Specific factors contributing to low sensitivity compared with the CDC ELISA could not be determined through further analysis of the limits and dilution end points of IgM detection.

• Authors: Jaimie S Robinson, David Featherstone, Ravi Vasanthapuram, Brad J Biggerstaff, Anita Desai, Nalini Ramamurty, Anwarul Haque Chowdhury, Hardeep S Sandhu, Kathleen F Cavallaro, Barbara W Johnson
• Journal: The American journal of tropical medicine and hygiene (Am J Trop Med Hyg) Vol. 83 Issue 5 Pg. 1146-55 (Nov 2010) ISSN: 1476-1645 [Electronic] United States
• PMID: 21036854 (Publication Type: Comparative Study, Evaluation Study, Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antibodies, Viral
• Immunoglobulin M
• Reagent Kits, Diagnostic

Topics

• Antibodies, Viral (blood, cerebrospinal fluid)
• Encephalitis Virus, Japanese (immunology)
• Encephalitis, Japanese (diagnosis, immunology)
• Enzyme-Linked Immunosorbent Assay (methods)
• Humans
• Immunoglobulin M (blood, cerebrospinal fluid)
• Reagent Kits, Diagnostic
• Sensitivity and Specificity