[Cell cycle arrest and apoptosis induced by arsenic trioxide in human lung cancer cell line].

Abstract

BACKGROUND:

To explore the potential therapeutic effect of arsenic trioxide(As₂O₃ ) on human lung cancer.

METHODS:

Cell growth curves, cell proliferation, cell cycle and apoptosis of human lung cancer cell line GLC-82 were detected by theMTT method and flowcytometry (FCM) .

RESULTS:

The data showed that As₂O₃ significantly inhibited proliferation of GLC-82 cells and the inhibiting effects had a dose-and time-dependence. The cell proliferation inhibition rate was 81. 05% when treated with 4. 0 Lmol/L As₂O₃ for 96 hours. The change of DNA content of GLC-82 cells indicated that As₂O₃ was able to block cell cycle progress in G2/M phase, with appearance of sub-G1 peak in a dose-dependent manner.

CONCLUSIONS:

As₂O₃ can effectively inhibit the proliferation of human lung cancer cell line GLC-82 and the possible mechanisms may be related to G2/M phase arrest and apoptosis induced by As₂O₃.

• Authors: J Dong, Y Wu, X Dong, L Xu, L Liu
• Journal: Zhongguo fei ai za zhi = Chinese journal of lung cancer (Zhongguo Fei Ai Za Zhi) Vol. 3 Issue 6 Pg. 435-7 (Dec 20 2000) ISSN: 1009-3419 [Print] China
• PMID: 21029577 (Publication Type: English Abstract, Journal Article)