Abstract | INTRODUCTION: Giardia intestinalis is a unicellular parasite of worldwide distribution. It causes an intestinal illness known as giardiasis, and it is probably the earliest diverging eukaryotic microorganism. Previously, changes have been reported in the expression of mRNAs at several stages of the life cycle; however specific enzymatic activity changes have not been explored. OBJECTIVE: MATERIALS AND METHODS: RESULTS: PFOR (139 kDa) and ADHE (97 kDa) proteins were detected in trophozoites, but not in cysts. During excystation, ADHE protein was detected after the first phase of induction, but the PFOR protein appeared only after the second phase. This indicated that both proteins were synthesized during excystation, although at different times. ADHE enzymatic activity was present only in trophozoites and not in cysts whereas GDH activity was detected in both stages. CONCLUSION: These results conclusively showed that PFOR and ADHE enzymes were translated during the excystation process and is strong evidence that active protein synthesis was occurring during excystation.
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Authors | Carlos Alberto Niño, Moisés Wasserman |
Journal | Biomedica : revista del Instituto Nacional de Salud
(Biomedica)
2010 Jan-Mar
Vol. 30
Issue 1
Pg. 32-8
ISSN: 2590-7379 [Electronic] Colombia |
PMID | 20890547
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Alcohol Dehydrogenase
- Pyruvate Synthase
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Topics |
- Alcohol Dehydrogenase
(biosynthesis)
- Giardia lamblia
(enzymology, growth & development)
- Pyruvate Synthase
(biosynthesis)
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