Daptomycin and rifampin alone and in combination prevent vascular graft biofilm formation and emergence of antibiotic resistance in a subcutaneous rat pouch model of staphylococcal infection.

Abstract

OBJECTIVE:

To investigate the efficacy of daptomycin and rifampin either alone or in combination in preventing prosthesis biofilm in a rat model of staphylococcal vascular graft infection.

DESIGN:

Prospective, randomised, controlled animal study.

MATERIALS:

Graft infections were established in the back subcutaneous tissue of adult male Wistar rats by implantation of Dacron prostheses followed by topical inoculation with 2×10(7) colony forming units of Staphylococcus aureus, strain Smith diffuse.

METHODS:

The study included a control group, a contaminated group that did not receive any antibiotic prophylaxis and three contaminated groups that received intra-peritoneal daptomycin, rifampin-soaked graft and daptomycin plus rifampin-soaked graft, respectively. Each group included 15 animals. The infection burden was evaluated by using sonication and quantitative agar culture. Moreover, an in vitro antibiotic susceptibility assay for S. aureus biofilms was performed to elucidate the same activity.

RESULTS:

When tested alone, daptomycin and rifampin showed good efficacies. Their combination showed efficacies significantly higher than that of each single compound. The in vitro studies showed that minimum inhibitory concentration and minimum bactericidal concentration values for daptomycin were lower in presence of rifampin. Daptomycin prevented the emergence of rifampin resistance.

CONCLUSION:

Daptomycin is an important candidate for prevention of staphylococcal biofilm-related infection and rifampin could serve as an interesting anti-staphylococcal antibiotic enhancer.

• Authors: O Cirioni, F Mocchegiani, R Ghiselli, C Silvestri, E Gabrielli, E Marchionni, F Orlando, D Nicolini, A Risaliti, A Giacometti
• Journal: European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery (Eur J Vasc Endovasc Surg) Vol. 40 Issue 6 Pg. 817-22 (Dec 2010) ISSN: 1532-2165 [Electronic] England
• PMID: 20869272 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2010 European Society for Vascular Surgery. Published by Elsevier Ltd. All rights reserved.

Chemical References

• Anti-Bacterial Agents
• Coated Materials, Biocompatible
• Polyethylene Terephthalates
• Daptomycin
• Rifampin

Topics

• Animals
• Anti-Bacterial Agents (administration & dosage)
• Antibiotic Prophylaxis
• Biofilms
• Blood Vessel Prosthesis (adverse effects, microbiology)
• Blood Vessel Prosthesis Implantation (adverse effects, instrumentation)
• Coated Materials, Biocompatible
• Colony Count, Microbial
• Daptomycin (administration & dosage)
• Disease Models, Animal
• Drug Resistance, Multiple, Bacterial
• Drug Therapy, Combination
• Male
• Microbial Sensitivity Tests
• Polyethylene Terephthalates
• Prospective Studies
• Prosthesis Design
• Prosthesis-Related Infections (microbiology, prevention & control)
• Random Allocation
• Rats
• Rats, Wistar
• Rifampin (administration & dosage)
• Staphylococcal Infections (microbiology, prevention & control)
• Staphylococcus aureus (drug effects, growth & development)