Abstract |
To evaluate the effect of thermoseed inductive heating on mammary orthotopic transplantation tumors and immunologic function in rats. Walker-256 tumor cells were inoculated subcutaneously into the mammary glands of Wistar rats. Rats were allocated to five treatment groups as follows: i) C group (control group); ii) M group (magnetic field group); iii) T group (thermoseed control group); iv) H1 group ( hyperthermia treatment, 45 degrees C for 30 min); v) H2 group ( hyperthermia treatment, 50-55 degrees C for 10 min). Immediately, 12 and 24 h after hyperthermia, two rats were sacrificed in each group for pathological and immunohistochemical examination of the expression of PCNA and HSP70. Tumor volume was measured and long-term survival was observed. The T lymphocyte subgroup IL-2 and IFN-gamma levels were measured in C, H1 and H2 groups. Both types of hyperthermia induced necrosis and apoptosis in the tumor tissue, decreased tumor volume (P<0.05), and increased survival time (P<0.01). The expression of PCNA and HSP70 in hyperthermia group was significantly different compared to the C, M and T groups (P<0.05), Hyperthermia increased CD4+ T lymphocytes and the levels of IL-2 and IFN-gamma (P<0.05). Both types of hyperthermia can suppress the growth of mammary tumors and improve immunological function of rats.
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Authors | Weiwei Ouyang, Fuping Gao, Lufang Wang, Xiaoxue Xie, Fenglin Lei, Jumei Zhou, Yuping Liao, Meizuo Zhong, Jintian Tang |
Journal | Oncology reports
(Oncol Rep)
Vol. 24
Issue 4
Pg. 973-9
(Oct 2010)
ISSN: 1791-2431 [Electronic] Greece |
PMID | 20811678
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- HSP70 Heat-Shock Proteins
- Interleukin-2
- Proliferating Cell Nuclear Antigen
- nickel copper alloy
- Copper
- Nickel
- Interferon-gamma
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Topics |
- Animals
- Cell Separation
- Copper
(therapeutic use)
- Enzyme-Linked Immunosorbent Assay
- Female
- Flow Cytometry
- HSP70 Heat-Shock Proteins
(biosynthesis)
- Hyperthermia, Induced
(methods)
- Immunohistochemistry
- Implants, Experimental
- Interferon-gamma
(blood)
- Interleukin-2
(blood)
- Magnetic Field Therapy
(methods)
- Mammary Neoplasms, Experimental
(blood, immunology, therapy)
- Nickel
(therapeutic use)
- Proliferating Cell Nuclear Antigen
(biosynthesis)
- Rats
- Rats, Wistar
- T-Lymphocytes
(immunology)
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