Induction of growth arrest by miR-542-3p that targets survivin.

Abstract

Survivin is a protein which functions as a mitotic regulator as well as apoptosis inhibitor. In this study, we show that introduction of synthetic miR-542-3p mimetic reduced both mRNA and protein levels of survivin. In A549 cells, luciferase reporter assay revealed that miR-542-3p targeted predicted binding sites in the 3'-untranslated region (3'-UTR) of survivin. We also demonstrate that ectopic expression of miR-542-3p inhibited cell proliferation by inducing Gap 1 (G1) and Gap 2/Mitosis (G2/M) cell cycle arrest. Collectively, these results suggest that survivin is a direct target of miR-542-3p and growth inhibition by miR-542-3p may have a potential utility as an anti-cancer therapy.

• Authors: Sena Yoon, Young-Chul Choi, Suman Lee, Yongsu Jeong, Jaeseung Yoon, Kwanghee Baek
• Journal: FEBS letters (FEBS Lett) Vol. 584 Issue 18 Pg. 4048-52 (Sep 24 2010) ISSN: 1873-3468 [Electronic] England
• PMID: 20728447 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Chemical References

• BIRC5 protein, human
• Inhibitor of Apoptosis Proteins
• MicroRNAs
• Microtubule-Associated Proteins
• Survivin

Topics

• Cell Cycle (genetics)
• Cell Line, Tumor
• Cell Proliferation
• Gene Expression Regulation, Neoplastic
• Humans
• Inhibitor of Apoptosis Proteins
• MicroRNAs (genetics, metabolism)
• Microtubule-Associated Proteins (genetics)
• Mitosis (genetics)
• Oligonucleotide Array Sequence Analysis
• Survivin