Abstract | BACKGROUND: METHODS: In the current study, we generated an irradiated tumor cell-based drug delivery system which uses irradiated tumor cells loaded with the chemotherapeutic drug, doxorubicin. RESULTS: We showed that incubation of murine ovarian cancer cells (MOSEC) with doxorubicin led to the intracellular uptake of the drug (MOSEC-dox cells) and the eventual death of the tumor cell. We then showed that doxorubicin loaded MOSEC-dox cells were able to deliver doxorubicin to MOSEC cells in vivo. Further characterization of the doxorubicin transfer revealed the involvement of cell contact. The irradiated form of the MOSEC-dox cells were capable of treating luciferase-expressing MOSEC tumor cells (MOSEC/luc) in C57BL/6 mice as well as in athymic nude mice resulting in improved survival compared to the non drug-loaded irradiated MOSEC cells. Furthermore, we showed that irradiated MOSEC-dox cells was more effective compared to an equivalent dose of doxorubicin in treating MOSEC/luc tumor-bearing mice. CONCLUSIONS: Thus, the employment of drug-loaded irradiated tumor cells represents a potentially innovative approach for the delivery of chemotherapeutic drugs for the control of ovarian tumors.
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Authors | Daejin Kim, Talia Hoory, Archana Monie, Annie Wu, Wei-Ting Hsueh, Sara I Pai, Chien-Fu Hung |
Journal | Journal of biomedical science
(J Biomed Sci)
Vol. 17
Pg. 61
(Jul 26 2010)
ISSN: 1423-0127 [Electronic] England |
PMID | 20659328
(Publication Type: Comparative Study, Evaluation Study, Journal Article, Research Support, N.I.H., Extramural)
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Chemical References |
- Drug Carriers
- Doxorubicin
- Luciferases
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Topics |
- Animals
- Doxorubicin
(metabolism, pharmacokinetics, therapeutic use)
- Drug Carriers
(therapeutic use)
- Drug Therapy
(methods)
- Female
- Flow Cytometry
- Luciferases
- Mice
- Mice, Inbred C57BL
- Ovarian Neoplasms
(drug therapy)
- Tumor Cells, Cultured
(metabolism, radiation effects)
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