Abstract | BACKGROUND AND AIMS: We undertook this study to investigate the effect of mesangial cells stimulated by immunoglobin A1 from IgAN on transforming growth factor-beta1 synthesis in podocytes. METHODS:
Jacalin affinity chromatography and Sephacryl S-200 molecular sieve chromatography were used to isolate IgA1 from blood of IgAN patients, which was then used as aggregated IgA1 (aIgA1). Podocytes were incubated with special mesangial medium. Real-time polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) were used, respectively, to measure TGF-beta1 mRNA expression and its protein concentration in medium. RESULTS: CONCLUSIONS: These data suggest that mesangial cells stimulated by IgA1 from IgAN patients may excrete some material to facilitate TGF-beta1 synthesis in podocytes through activating renin-angiotensin system by cross-talk.
|
Authors | Cheng Wang, Xun Liu, Hui Peng, Ying Tang, Hua Tang, Zhujiang Chen, Tanqi Lou, Hui Zhang |
Journal | Archives of medical research
(Arch Med Res)
Vol. 41
Issue 4
Pg. 255-60
(May 2010)
ISSN: 1873-5487 [Electronic] United States |
PMID | 20637368
(Publication Type: Journal Article)
|
Copyright | Copyright 2010 IMSS. All rights reserved. |
Chemical References |
- Culture Media
- DNA Primers
- Immunoglobulin A
- Transforming Growth Factor beta1
|
Topics |
- Animals
- Base Sequence
- Culture Media
- DNA Primers
- Enzyme-Linked Immunosorbent Assay
- Glomerular Mesangium
(drug effects, metabolism, pathology)
- Glomerulonephritis, IGA
(metabolism, pathology)
- Humans
- Immunoglobulin A
(pharmacology)
- Mice
- Podocytes
(metabolism)
- Polymerase Chain Reaction
- Renin-Angiotensin System
- Transforming Growth Factor beta1
(biosynthesis)
- Up-Regulation
(drug effects)
|