Lipopeptide-adjuvanted respiratory syncytial virus virosomes: A safe and immunogenic non-replicating vaccine formulation.

Abstract

Respiratory syncytial virus (RSV) causes severe respiratory disease in children and the elderly. There is no registered RSV vaccine. Early experimental non-replicating vaccines have been found to exacerbate RSV symptoms upon infection causing enhanced respiratory disease. Here we show that immunization of mice with reconstituted virosomes produced from RSV envelopes and containing the lipopeptide adjuvant (P3CSK4), induces high-titer virus-neutralizing antibodies, and the secretion of IFN-gamma through both MHC-I and MHC-II presentation of antigen, with a balanced Th1/Th2 profile. Immunization with RSV virosomes provides sterilizing immunity to virus challenge in mice and cotton rats, while not producing symptoms of enhanced disease. Therefore, these virosomes represent a promising candidate inactivated RSV vaccine formulation.

• Authors: Toon Stegmann, Tobias Kamphuis, Tjarko Meijerhof, Ellen Goud, Aalzen de Haan, Jan Wilschut
• Journal: Vaccine (Vaccine) Vol. 28 Issue 34 Pg. 5543-50 (Aug 02 2010) ISSN: 1873-2518 [Electronic] Netherlands
• PMID: 20600502 (Publication Type: Journal Article)
• Copyright: Copyright 2010 Elsevier Ltd. All rights reserved.

Chemical References

• Adjuvants, Immunologic
• Antibodies, Neutralizing
• Antibodies, Viral
• Lipopeptides
• Respiratory Syncytial Virus Vaccines
• Viral Envelope Proteins
• Virosomes
• Interferon-gamma

Topics

• Adjuvants, Immunologic (pharmacology)
• Animals
• Antibodies, Neutralizing (blood)
• Antibodies, Viral (blood)
• Antigen Presentation
• Female
• Interferon-gamma (immunology)
• Lipopeptides (immunology)
• Mice
• Mice, Inbred BALB C
• Neutralization Tests
• Rats
• Respiratory Syncytial Virus Infections (immunology, prevention & control)
• Respiratory Syncytial Virus Vaccines (immunology)
• Respiratory Syncytial Viruses (immunology)
• Viral Envelope Proteins (immunology)
• Virosomes (immunology)