Abstract | BACKGROUND:
Glycodelin is a glycoprotein with a molecular weight of 28 kDa. Due to its different glycosylation, several glycodelin molecules have been described, including glycodelin-A (amniotic fluid). The precise function of glycodelin is still not well understood, although immunosuppressive, contraceptive and marker of morphological differentiation roles have been demonstrated. The aim of this study was to assess the expression of glycodelin in malignant tumors of the ovary correlated to grading and staging. MATERIALS AND METHODS:
Paraffin sections of 187 ovarian cancer specimens (including 132 serous, 22 endometrioid, 17 mucinous, 12 clear cell and 4 borderline tumors) were analyzed with a monoclonal antibody GdA (MAb) and a peptide polyclonal antibody (PAb) against glycodelin. The intensity and distribution of the specific immunohistochemical staining reaction was evaluated by using a semi-quantitative method (immunoreactive score (IRS)). RESULTS: We identified significant changes in glycodelin-A expression corresponding to grading and staging. Analysis of glycodelin expression with the PAb did not result in significant differences. Glycodelin-A staining was significantly reduced in G2 carcinomas compared to G1 ovarian cancer tissue. Moreover, ovarian cancer of surgical stage FIGO III-IV demonstrated a significant lower glycodelin-A expression compared to FIGO I-II stage tumors. CONCLUSION:
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Authors | Alexandra Tsviliana, Doris Mayr, Christina Kuhn, Susanne Kunze, Ioannis Mylonas, Udo Jeschke, Klaus Friese |
Journal | Anticancer research
(Anticancer Res)
Vol. 30
Issue 5
Pg. 1637-40
(May 2010)
ISSN: 1791-7530 [Electronic] Greece |
PMID | 20592354
(Publication Type: Journal Article)
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Chemical References |
- Antibodies, Monoclonal
- Glycodelin
- Glycoproteins
- Immunosuppressive Agents
- PAEP protein, human
- Pregnancy Proteins
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Topics |
- Antibodies, Monoclonal
(metabolism)
- Carcinoma
(metabolism)
- Female
- Gene Expression Profiling
- Gene Expression Regulation, Neoplastic
- Glycodelin
- Glycoproteins
(biosynthesis)
- Humans
- Immunohistochemistry
(methods)
- Immunosuppressive Agents
(metabolism)
- Models, Statistical
- Neoplasm Staging
(methods)
- Ovarian Neoplasms
(metabolism)
- Pregnancy Proteins
(biosynthesis)
- Specimen Handling
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