Abstract |
Tm-1 is a gene that provides resistance to tomato plants against tobacco mosaic virus (TMV) infection. In tomato cells carrying the Tm-1 gene, multiplication of TMV is inhibited. From previous analysis of resistance-breaking mutants, the involvement of the 130- and 180-kDa proteins, putative viral replicases, in the resistance conferred by the Tm-1 gene was suggested. When wild-type TMV RNA was co-inoculated with a resistance-breaking mutant RNA, replication of the wild-type TMV genomic RNA could not be rescued by the 130- and 180-kDa proteins of a resistance-breaking strain, Lta1. To investigate how the putative resistance factor interacts with the 130- and 180-kDa proteins, we expressed the wild-type TMV protein sequence that is associated with the resistance-breaking phenomenon as part of a recombinant virus derived from Lta1 in Tm-1/ Tm-1 protoplasts. No specific degradation of wild-type TMV protein sequences was observed, suggesting that the mechanism of the resistance does not involve the instability of a viral protein.
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Authors | R Yamafuji, Y Watanabe, T Meshi, Y Okada |
Journal | Virology
(Virology)
Vol. 183
Issue 1
Pg. 99-105
(Jul 1991)
ISSN: 0042-6822 [Print] United States |
PMID | 2053299
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Capsid Proteins
- H protein, Tobacco mosaic virus
- RNA, Viral
- Recombinant Fusion Proteins
- Viral Proteins
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Topics |
- Amino Acid Sequence
- Base Sequence
- Capsid Proteins
- Genes, Plant
- Molecular Sequence Data
- Molecular Weight
- Plant Diseases
- Protoplasts
(microbiology)
- RNA, Viral
(biosynthesis)
- Recombinant Fusion Proteins
(biosynthesis, genetics)
- Recombination, Genetic
- Tobacco Mosaic Virus
(genetics)
- Viral Proteins
(biosynthesis, genetics)
- Virus Replication
(genetics)
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