Abstract | BACKGROUND: METHODOLOGY/PRINCIPAL FINDINGS: To test this hypothesis, rats receiving paw incision surgery were used as a model of acute postoperative pain that spontaneously resolves. We first characterized the concentration of ECBs and localization of CB(1) and CB(2) receptors in the spinal cord following paw incision. We then administered concomitant CB(1) and CB(2) receptor antagonists/inverse agonists (AM281 and AM630, 1 mg x kg(-1) each, i.p.) during the acute phase of paw incision-induced mechanical allodynia and evaluated the expression of glial cell markers and phosphorylated p38 (a MAPK associated with inflammation) in the lumbar dorsal horn. Dual blockade of CB(1) and CB(2) receptor signaling prevented the resolution of postoperative allodynia and resulted in persistent over-expression of spinal Glial Fibrillary Acidic Protein (GFAP, an astrocytic marker) and phospho-p38 in astrocytes. We provide evidence for the functional significance of these astrocytic changes by demonstrating that intrathecal administration of propentofylline (50 microg, i.t.) attenuated both persistent behavioral hypersensitivity and over-expression of GFAP and phospho-p38 in antagonist-treated animals. CONCLUSIONS/SIGNIFICANCE: Our results demonstrate that endocannabinoid signaling via CB(1) and CB(2) receptors is necessary for the resolution of paw incision-induced behavioral hypersensitivity and for the limitation of pro-inflammatory signaling in astrocytes following surgical insult. Our findings suggest that therapeutic strategies designed to enhance endocannabinoid signaling may prevent patients from developing persistent or chronic pain states following surgery.
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Authors | Matthew S Alkaitis, Carlos Solorzano, Russell P Landry, Daniele Piomelli, Joyce A DeLeo, E Alfonso Romero-Sandoval |
Journal | PloS one
(PLoS One)
Vol. 5
Issue 5
Pg. e10891
(May 28 2010)
ISSN: 1932-6203 [Electronic] United States |
PMID | 20531936
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Aif1 protein, rat
- Biomarkers
- Calcium-Binding Proteins
- Cannabinoid Receptor Modulators
- Endocannabinoids
- Glial Fibrillary Acidic Protein
- Microfilament Proteins
- Receptor, Cannabinoid, CB1
- Receptor, Cannabinoid, CB2
- Xanthines
- propentofylline
- p38 Mitogen-Activated Protein Kinases
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Topics |
- Animals
- Astrocytes
(drug effects, metabolism, pathology)
- Behavior, Animal
(drug effects)
- Biomarkers
(metabolism)
- Calcium-Binding Proteins
(metabolism)
- Cannabinoid Receptor Modulators
(metabolism)
- Endocannabinoids
- Glial Fibrillary Acidic Protein
(metabolism)
- Male
- Microfilament Proteins
- Neuroglia
(drug effects, metabolism)
- Pain, Postoperative
(enzymology, pathology)
- Phosphorylation
(drug effects)
- Rats
- Rats, Sprague-Dawley
- Receptor, Cannabinoid, CB1
(antagonists & inhibitors, metabolism)
- Receptor, Cannabinoid, CB2
(antagonists & inhibitors, metabolism)
- Spinal Cord
(metabolism, pathology)
- Xanthines
(pharmacology)
- p38 Mitogen-Activated Protein Kinases
(metabolism)
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