FGF-2, TGFbeta-1, PDGF-A and respective receptors expression in pleomorphic adenoma myoepithelial cells: an in vivo and in vitro study.

Abstract

UNLABELLED:

Myoepithelial cells have an important role in salivary gland tumor development, contributing to a low grade of aggressiveness of these tumors. Normal myoepithelial cells are known by their suppressor function presenting increased expression of extracellular matrix genes and protease inhibitors. The importance of stromal cells and growth factors during tumor initiation and progression has been highlighted by recent literature. Many tumors result from the alteration of paracrine growth factors pathways. Growth factors mediate a wide variety of biological processes such as development, tissue repair and tumorigenesis, and also contribute to cellular proliferation and transformation in neoplastic cells.

OBJECTIVES:

This study evaluated the expression of fibroblast growth factor-2 (FGF-2), transforming growth factor beta-1 (TGFbeta-1), platelet-derived growth factor-A (PDGF-A) and their respective receptors (FGFR-1, FGFR-2, TGFbetaR-II and PDGFR-alpha) in myoepithelial cells from pleomorphic adenomas (PA) by in vivo and in vitro experiments.

MATERIAL AND METHODS:

Serial sections were obtained from paraffin-embedded PA samples obtained from the school's files. Myoepithelial cells were obtained from explants of PA tumors provided by surgery from different donors. Immunohistochemistry, cell culture and immunofluorescence assays were used to evaluate growth factor expression.

RESULTS:

The present findings demonstrated that myoepithelial cells from PA were mainly positive to FGF-2 and FGFR-1 by immunohistochemistry and immunofluorescence. PDGF-A and PDGFR-alpha had moderate expression by immunohistochemistry and presented punctated deposits throughout cytoplasm of myoepithelial cells. FGFR-2, TGFbeta-1 and TGFbetaR-II were negative in all samples.

CONCLUSIONS:

These data suggested that FGF-2 compared to the other studied growth factors has an important role in PA benign myoepithelial cells, probably contributing to proliferation of these cells through the FGFR-1.

• Authors: Lucyene Miguita, Elizabeth Ferreira Martinez, Ney Soares de Araújo, Vera Cavalcanti de Araújo
• Journal: Journal of applied oral science : revista FOB (J Appl Oral Sci) 2010 Jan-Feb Vol. 18 Issue 1 Pg. 83-91 ISSN: 1678-7765 [Electronic] Brazil
• PMID: 20379686 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Actins
• Calcium-Binding Proteins
• Keratin-7
• Microfilament Proteins
• Muscle Proteins
• Platelet-Derived Growth Factor
• Receptors, Transforming Growth Factor beta
• Transforming Growth Factor beta1
• Vimentin
• calponin
• platelet-derived growth factor A
• Fibroblast Growth Factor 2
• FGFR1 protein, human
• FGFR2 protein, human
• Receptor, Fibroblast Growth Factor, Type 1
• Receptor, Fibroblast Growth Factor, Type 2
• Receptor, Platelet-Derived Growth Factor alpha
• Protein Serine-Threonine Kinases
• Receptor, Transforming Growth Factor-beta Type II

Topics

• Actins (analysis)
• Adenoma, Pleomorphic (pathology)
• Adult
• Calcium-Binding Proteins (analysis)
• Cell Nucleus (ultrastructure)
• Cells, Cultured
• Cytoplasm (ultrastructure)
• Epithelial Cells (pathology)
• Female
• Fibroblast Growth Factor 2 (analysis)
• Fluorescent Antibody Technique
• Humans
• Immunohistochemistry
• Keratin-7 (analysis)
• Lip Neoplasms (pathology)
• Male
• Microfilament Proteins (analysis)
• Muscle Cells (pathology)
• Muscle Proteins (analysis)
• Muscle, Smooth (pathology)
• Palatal Neoplasms (pathology)
• Platelet-Derived Growth Factor (analysis)
• Protein Serine-Threonine Kinases (analysis)
• Receptor, Fibroblast Growth Factor, Type 1 (analysis)
• Receptor, Fibroblast Growth Factor, Type 2 (analysis)
• Receptor, Platelet-Derived Growth Factor alpha (analysis)
• Receptor, Transforming Growth Factor-beta Type II
• Receptors, Transforming Growth Factor beta (analysis)
• Salivary Gland Neoplasms (pathology)
• Transforming Growth Factor beta1 (analysis)
• Vimentin (analysis)
• Young Adult