Changes in function of HIV-specific T-cell responses with increasing time from infection.

Abstract

Recently HIV-infected individuals have virus-specific responses characterized by IFN-gamma/IL-2 secretion and proliferation rarely seen in chronic infection. To investigate the timing of loss of HIV-specific T-cell function, we screened cells from 59 treatment-naïve HIV-infected individuals with known dates of infection for proteome-wide responses secreting IFN-gamma/IL-2 and IFN-gamma alone by ELISPOT. HIV peptide-specific proliferation was assessed by carboxyfluorescein diacetate succinimidyl ester (CFSE) dilution. The contribution of IFN-gamma/IL-2 and IFN-gamma-only secretion to the total HIV-specific response was compared in subjects infected <6, 6-12, and 12-36 mo earlier. The frequency of IFN-gamma/IL-2-secreting cells fell, while that of IFN-gamma-only secretion rose with time from infection. HIV peptide-specific proliferative responses were almost exclusively mediated by CD8(+) T cells, and were significantly lower in cells obtained from the 12-36 mo versus < 6 mo post-infection groups. By the second year of infection there was a significant difference in these functions compared to those assessed within 6 mo.

• Authors: Michel L Ndongala, Philomena Kamya, Salix Boulet, Yoav Peretz, Danielle Rouleau, Cécile Tremblay, Roger Leblanc, Pierre Côté, Jean-Guy Baril, RéJean Thomas, Sylvie Vézina, Mohamed R Boulassel, Jean-Pierre Routy, Rafick P Sékaly, Nicole F Bernard
• Journal: Viral immunology (Viral Immunol) Vol. 23 Issue 2 Pg. 159-68 (Apr 2010) ISSN: 1557-8976 [Electronic] United States
• PMID: 20373996 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Human Immunodeficiency Virus Proteins
• Interleukin-2
• Peptides
• Proteome
• Interferon-gamma

Topics

• Adult
• CD8-Positive T-Lymphocytes (immunology, metabolism)
• Female
• HIV Infections (immunology)
• HIV-1 (immunology)
• Human Immunodeficiency Virus Proteins (immunology)
• Humans
• Interferon-gamma (biosynthesis)
• Interleukin-2 (biosynthesis)
• Lymphocyte Count
• Male
• Peptides (immunology)
• Proteome
• T-Cell Antigen Receptor Specificity
• T-Lymphocytes (immunology, metabolism)
• Time Factors