Inorganic polyphosphate induces osteoblastic differentiation.

Abstract

Inorganic polyphosphate [Poly(P)] is especially prevalent in osteoblasts. We tested the hypothesis that Poly(P) stimulates osteoblastic differentiation and polyphosphate metabolism for bone formation. The osteoblast-like cell line, MC 3T3-E1, was cultured with Poly(P), and gene expression was evaluated by real-time reverse-transcription polymerase chain-reaction. Phosphatase activity and extracellular matrix mineralization were also determined. The role of Poly(P) was assessed in a beagle dog alveolar bone regeneration model. Poly(P) increased osteocalcin, osterix, bone sialoprotein, and tissue non-specific alkaline phosphatase gene expression, with a high level of end-polyphosphatase activity, resulting in low-chain-length Poly(P), inorganic pyrophosphate, and inorganic phosphate production. MC3T3-E1 cells differentiated into mature osteoblasts and showed expression of ectonucleotide pyrophosphatase phosphodiesterase 1, while mouse progressive ankylosis gene expression remained unchanged. Promotion of alveolar bone regeneration was observed in Poly(P)-treated beagle dogs. These findings suggest that Poly(P) induces osteoblastic differentiation and bone mineralization, and acts as a resource for mineralization.

• Authors: Y Usui, T Uematsu, T Uchihashi, M Takahashi, M Takahashi, M Ishizuka, R Doto, H Tanaka, Y Komazaki, M Osawa, K Yamada, M Yamaoka, K Furusawa
• Journal: Journal of dental research (J Dent Res) Vol. 89 Issue 5 Pg. 504-9 (May 2010) ISSN: 1544-0591 [Electronic] United States
• PMID: 20332330 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Dental Enamel Proteins
• Diphosphates
• Ibsp protein, mouse
• Integrin-Binding Sialoprotein
• Phosphates
• Polyphosphates
• Sialoglycoproteins
• Sp7 Transcription Factor
• Sp7 protein, mouse
• Transcription Factors
• enamel matrix proteins
• Osteocalcin
• Alkaline Phosphatase
• Phosphoric Diester Hydrolases
• ectonucleotide pyrophosphatase phosphodiesterase 1
• Acid Anhydride Hydrolases
• Pyrophosphatases
• endopolyphosphatase

Topics

• 3T3 Cells
• Acid Anhydride Hydrolases (analysis)
• Alkaline Phosphatase (analysis)
• Alveolar Bone Loss (surgery)
• Alveolar Process (drug effects)
• Animals
• Bone Regeneration (drug effects)
• Calcification, Physiologic (drug effects)
• Cell Differentiation (drug effects)
• Dental Enamel Proteins (therapeutic use)
• Diphosphates (analysis)
• Dogs
• Extracellular Matrix (drug effects)
• Furcation Defects (surgery)
• Integrin-Binding Sialoprotein
• Male
• Mice
• Osteoblasts (drug effects)
• Osteocalcin (analysis)
• Phenotype
• Phosphates (analysis)
• Phosphoric Diester Hydrolases (analysis)
• Polyphosphates (pharmacology, therapeutic use)
• Pyrophosphatases (analysis)
• Sialoglycoproteins (analysis)
• Sp7 Transcription Factor
• Transcription Factors (analysis)
• Zinc Fingers (drug effects)