Abstract | AIM: MAIN METHODS: Uptake characteristics of [(14)C] nicotinamide were assessed by a conventional method with primary cultured mouse astrocytes. Cell viability and PARP-1 activity were determined with intracellular LDH activity and immunocytochemical detection of PAR accumulation, respectively. KEY FINDINGS: SIGNIFICANCE: These findings suggest that nicotinamide has a protective effect against PARP-1-induced astrocyte death and that its transporter-mediated uptake, which is extracellular pH-sensitive and common to N-methylnicotinamide, is critical for prevention of PARP-1-triggered cell death.
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Authors | Eri Suzuki, Hiroto Okuda, Kentaro Nishida, Sadaki Fujimoto, Kazuki Nagasawa |
Journal | Life sciences
(Life Sci)
Vol. 86
Issue 17-18
Pg. 676-82
(Apr 24 2010)
ISSN: 1879-0631 [Electronic] Netherlands |
PMID | 20188745
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Vitamin B Complex
- Niacinamide
- Parp1 protein, mouse
- Poly (ADP-Ribose) Polymerase-1
- Poly(ADP-ribose) Polymerases
- N-methylnicotinamide
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Topics |
- Animals
- Astrocytes
(drug effects, metabolism)
- Biological Transport
- Cell Death
(drug effects)
- Cell Survival
(drug effects)
- Hydrogen-Ion Concentration
- Mice
- Niacinamide
(analogs & derivatives, pharmacokinetics, pharmacology)
- Poly (ADP-Ribose) Polymerase-1
- Poly(ADP-ribose) Polymerases
(metabolism)
- Temperature
- Time Factors
- Vitamin B Complex
(pharmacokinetics, pharmacology)
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