Hydrazone ligation strategy to assemble multifunctional viral nanoparticles for cell imaging and tumor targeting.

Abstract

Multivalent nanoparticle platforms are attractive for biomedical applications because of their improved target specificity, sensitivity, and solubility. However, their controlled assembly remains a considerable challenge. An efficient hydrazone ligation chemistry was applied to the assembly of Cowpea mosaic virus (CPMV) nanoparticles with individually tunable levels of a VEGFR-1 ligand and a fluorescent PEGylated peptide. The nanoparticles recognized VEGFR-1 on endothelial cell lines and VEGFR1-expressing tumor xenografts in mice, validating targeted CPMV as a nanoparticle platform in vivo.

• Authors: Florence M Brunel, John D Lewis, Giuseppe Destito, Nicole F Steinmetz, Marianne Manchester, Heidi Stuhlmann, Philip E Dawson
• Journal: Nano letters (Nano Lett) Vol. 10 Issue 3 Pg. 1093-7 (Mar 10 2010) ISSN: 1530-6992 [Electronic] United States
• PMID: 20163184 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Hydrazones

Topics

• Animals
• Drug Delivery Systems (methods)
• HT29 Cells
• Humans
• Hydrazones (chemistry)
• Image Enhancement (methods)
• Mice
• Nanostructures (chemistry)
• Virion (chemistry)