Abstract | OBJECTIVE: METHODS: The expression levels of ghrelin, GHSR-1a mRNA were determined by SYBR green real-time fluorescent quantitative PCR. The gsp mutations in 43 cases of human GH-secreting pituitary adenomas were detected using PCR- DNA direct sequencing analysis. Clinical data were obtained from the medical records of 43 acromegalic patients who had GH and IGF-1 assays in the same laboratory. RESULTS: The expression of GHSR-1a correlated positvely with tumor size (R=0.411, p=0.006) and invasiveness (p<0.05). In contrast, ghrelin mRNA levels correlated positively only with tumor size (R=0.331, p=0.030) but not with tumor invasiveness (p>0.05). The expression level of GHSR-1a mRNA was significantly higher in gsp positive adenomas than in negative adenomas (p<0.05). Whereas, there was no significant difference in the expression of ghrelin mRNA between gsp mutation-positive and -negative adenomas (p>0.05). Additionally there was a significant positve correlation between the ghrelin and GHSR-1a mRNA expression levels in gsp-positive (R=0.553, p=0.040) or -negative adenomas (R=0.489, p=0.007). CONCLUSIONS:
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Authors | Tongjiang Xu, Fei Ye, Baofeng Wang, Chunlei Tian, Sheng Wang, Kai Shu, Dongsheng Guo, Ting Lei |
Journal | Neuro endocrinology letters
(Neuro Endocrinol Lett)
Vol. 31
Issue 1
Pg. 147-54
( 2010)
ISSN: 0172-780X [Print] Sweden |
PMID | 20150876
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- GTP-Binding Protein alpha Subunits
- Ghrelin
- RNA, Messenger
- Receptors, Ghrelin
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Topics |
- Acromegaly
(etiology, genetics)
- Adenoma
(complications, genetics, metabolism, pathology)
- Adult
- Female
- GTP-Binding Protein alpha Subunits
(genetics)
- Ghrelin
(genetics, metabolism)
- Growth Hormone-Secreting Pituitary Adenoma
(complications, genetics, metabolism, pathology)
- Humans
- Male
- Middle Aged
- Mutation
- RNA, Messenger
(metabolism)
- Receptors, Ghrelin
(genetics, metabolism)
- Retrospective Studies
- Tumor Burden
- Up-Regulation
- Young Adult
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