Screening of DNA aptamer which binds to alpha-synuclein.

Abstract

Alpha-synuclein is a native, unfolded protein that causes several neurodegenerative diseases such as dementia with Lewy bodies and Parkinson's disease. We have now identified the first DNA aptamers against alpha-synuclein using native PAGE applied to the SELEX method. We call this aptamer "M5-15"; it is the alpha-synuclein-bound aptamer and was isolated after four cycles of screening. M5-15 is composed of three stem-loop structures that may play an important role in the binding to alpha-synuclein. Moreover, M5-15 specifically binds to the alpha-synuclein monomer and oligomer. We expect that this aptamer will become a useful tool in alpha-synuclein analysis and diagnosis.

• Authors: Kaori Tsukakoshi, Ryuichi Harada, Koji Sode, Kazunori Ikebukuro
• Journal: Biotechnology letters (Biotechnol Lett) Vol. 32 Issue 5 Pg. 643-8 (May 2010) ISSN: 1573-6776 [Electronic] Netherlands
• PMID: 20111982 (Publication Type: Journal Article)

Chemical References

• Aptamers, Nucleotide
• alpha-Synuclein

Topics

• Aptamers, Nucleotide (isolation & purification, metabolism)
• Drug Evaluation, Preclinical (methods)
• Electrophoresis, Polyacrylamide Gel
• Electrophoretic Mobility Shift Assay
• Humans
• Protein Binding
• alpha-Synuclein (metabolism)