Abstract | PURPOSE: METHODS: RESULTS: The density of TAMs increased from 4 to 12 weeks of age in mice with small to medium tumors (P = 0.037) and remained stable in the later stages of disease (i.e., 16 weeks old with large tumors; P = 0.20). In 16-week-old mice, 38% (2.5 +/- 3.2 cells per 400x high-power field) of TAMs were GFP-positive, bone marrow-derived macrophages. Total TAM depletion was associated with a significant decrease in the expression levels of MMP-9 (P = 0.014) and mature vessels (P < 0.001) and a nonsignificant decrease in the density of neovessels (P = 0.94). The density of M2-polarized TAMs did not change significantly after TAM depletion (P = 0.68). After M1-polarized TAM depletion, the tumor burden increased (P = 0.056). CONCLUSIONS: This work extends understanding of the complex role that macrophages play in retinoblastoma. Macrophage modulation in the tumor microenvironment is a critical factor in retinoblastoma tumor progression.
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Authors | Yolanda Piña, Hinda Boutrid, Timothy G Murray, Martine J Jager, Colleen M Cebulla, Amy Schefler, Long V Ly, Armando Alegret, Magda Celdran, William Feuer, Maria-Elena Jockovich |
Journal | Investigative ophthalmology & visual science
(Invest Ophthalmol Vis Sci)
Vol. 51
Issue 5
Pg. 2671-7
(May 2010)
ISSN: 1552-5783 [Electronic] United States |
PMID | 20053982
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Matrix Metalloproteinase 9
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Topics |
- Animals
- Bone Marrow Cells
(cytology)
- Bone Marrow Transplantation
- Cell Count
- Disease Models, Animal
- Disease Progression
- Fluorescent Antibody Technique, Indirect
- Humans
- Macrophages
(physiology)
- Matrix Metalloproteinase 9
(metabolism)
- Mice
- Mice, Transgenic
- Microglia
(cytology)
- Neovascularization, Pathologic
(pathology)
- Retinal Neoplasms
(blood supply, pathology)
- Retinoblastoma
(blood supply, pathology)
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