Cross, but not direct, presentation of cell-associated virus antigens by spleen macrophages is influenced by their differentiation state.

Abstract

The initiation of T-cell immune responses requires professional antigen-presenting cells. Emerging data point towards an important role for macrophages (Mphi) in the priming of naïve T cells. In this study we analyzed the efficiency and the mechanisms by which Mphi derived from spleen (Sp-Mphi) or bone marrow (BM-Mphi) present Lymphocytic choriomeningitis virus (LCMV) antigens to epitope-specific T cells. We demonstrate that because of phagosomal maturation, Sp-Mphi downregulate their ability to cross-present cell-associated, but not soluble, antigens, as they are further differentiated in culture without altering their capacity to directly present virus antigens after infection. We propose that Sp-Mphi are extremely efficient at direct and cross-presentation. However, if these cells undergo further M-CSF-dependent maturation, they will adapt to be more scavenger and phagocytic and concurrently reduce their cross-presenting capacity. Accordingly, Sp-Mphi can have an important role in regulating T-cell responses through cross-presentation depending on their differentiation state.

• Authors: Attiya Alatery, Sarah Siddiqui, Matthew Chan, Agnieszka Kus, Elaine O Petrof, Sameh Basta
• Journal: Immunology and cell biology (Immunol Cell Biol) Vol. 88 Issue 1 Pg. 3-12 (Jan 2010) ISSN: 1440-1711 [Electronic] United States
• PMID: 19935765 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Viral
• Epitopes, T-Lymphocyte

Topics

• Animals
• Antigen Presentation
• Antigens, Viral (immunology)
• Bone Marrow (immunology)
• Cell Differentiation
• Cells, Cultured
• Cross-Priming
• Epitopes, T-Lymphocyte (immunology)
• Humans
• Lymphocytic choriomeningitis virus (immunology)
• Macrophages (cytology, immunology)
• Mice
• Mice, Inbred C57BL
• Phagocytosis
• Spleen (cytology, immunology)
• T-Lymphocytes (immunology)