Decreased expression of microRNA-199b increases protein levels of SET (protein phosphatase 2A inhibitor) in human choriocarcinoma.

Abstract

We compared microRNA profiles between choriocarcinoma and non-cancerous trophoblasts, and revealed that miR-199b was underexpressed in choriocarcinoma. By computational prediction and microarray studies, SET (protein phosphatase 2A inhibitor) was shown to be one of the target genes regulated by miR-199b. Ectopic expression of miR-199b inhibited endogenous SET protein levels and the activity of the luciferase reporter containing the 3'-UTR of SET. Further comparisons of formalin-fixed paraffin-embedded human choriocarcinoma, mole, and non-cancer trophoblast tissues confirmed the initial findings of low miR-199b expression and SET upregulation in choriocarcinomas, suggesting that microRNA-dysregulated SET protein may account for the rapid growth seen with choriocarcinomas.

• Authors: Angel Chao, Chia-Lung Tsai, Pei-Chi Wei, Swei Hsueh, An-Shine Chao, Chin-Jung Wang, Chi-Neu Tsai, Yun-Shien Lee, Tzu-Hao Wang, Chyong-Huey Lai
• Journal: Cancer letters (Cancer Lett) Vol. 291 Issue 1 Pg. 99-107 (May 01 2010) ISSN: 1872-7980 [Electronic] Ireland
• PMID: 19900756 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: 2009 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• DNA-Binding Proteins
• Histone Chaperones
• MicroRNAs
• SET protein, human
• Transcription Factors
• mirn199 microRNA, human

Topics

• Cell Line, Tumor
• Cell Proliferation
• Choriocarcinoma (chemistry, genetics, pathology)
• DNA-Binding Proteins
• Female
• Histone Chaperones (analysis, genetics, physiology)
• Humans
• MicroRNAs (analysis, physiology)
• Pregnancy
• Transcription Factors (analysis, genetics, physiology)
• Trophoblasts (chemistry)
• Uterine Neoplasms (chemistry, genetics, pathology)