Interferon regulatory factor-2 induces megakaryopoiesis in mouse bone marrow hematopoietic cells.

Abstract

Megakaryopoiesis is associated with inflammatory reactions. To investigate the role of interferon regulatory factors (IRFs) in inflammation-associated megakaryopoiesis, mouse bone marrow hematopoietic stem cells (HSCs) were analyzed. IFN-gamma treatment induced IRF-2 expression as well as the expression of CD41 and IRF-1 in HSCs. An in vitro clonogenic assay showed that IRF-2- but not IRF-1-overexpressing cells increased the number of megakaryocytic colonies. IRF-2 transfection up-regulated CD41 promoter activity in hematopoietic cell lines. The number of CD41-positive bone marrow cells increased in mice injected with IRF-2-expressing bone marrow cells. These findings suggest that IRF-2 plays an important role in megakaryopoiesis in inflammatory states.

• Authors: Atsuko Masumi, Isao Hamaguchi, Madoka Kuramitsu, Takuo Mizukami, Kazuya Takizawa, Haruka Momose, Seishiro Naito, Kazunari Yamaguchi
• Journal: FEBS letters (FEBS Lett) Vol. 583 Issue 21 Pg. 3493-500 (Nov 03 2009) ISSN: 1873-3468 [Electronic] England
• PMID: 19818776 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Interferon Regulatory Factor-2
• Platelet Membrane Glycoprotein IIb
• Interferon-gamma

Topics

• Animals
• Bone Marrow
• Cell Differentiation (drug effects)
• Cell Line
• Hematopoietic Stem Cells (cytology, metabolism)
• Humans
• Inflammation (metabolism)
• Interferon Regulatory Factor-2 (genetics, metabolism)
• Interferon-gamma (pharmacology)
• Megakaryocytes (cytology, metabolism)
• Mice
• Mice, Inbred C57BL
• Platelet Membrane Glycoprotein IIb (genetics, metabolism)
• Promoter Regions, Genetic (genetics)
• Rats
• Transcriptional Activation (drug effects)