Atromentin-induced apoptosis in human leukemia U937 cells.

Abstract

In the course of screening for apoptotic substances that induce apoptosis in human leukemia U937 cells, a fungal strain, F000487, which exhibits potent inducible activity, was selected. The active compound was purified from an ethyl acetate extract of the microorganism by Sep-pak C18 column chromatography and HPLC, and was identified as atromentin by spectroscopic methods. This compound induced caspase-3 processing in human leukemia U937 cells. The caspase-3 and poly (ADP-ribose) polymerase (PARP) were induced by atromentin in a dose-dependent manner. Furthermore, DNA fragmentation was also induced by this compound in a dose-dependent manner. These results show that atromentin potently induces apoptosis in U937 cells and that atromentin-induced apoptosis is related to the selective activation of caspases.

• Authors: Jin Hee Kim, Choong Hwan Lee
• Journal: Journal of microbiology and biotechnology (J Microbiol Biotechnol) Vol. 19 Issue 9 Pg. 946-50 (Sep 2009) ISSN: 1017-7825 [Print] Korea (South)
• PMID: 19809251 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Benzoquinones
• DNA, Neoplasm
• Phenols
• Soil
• atromentin
• Caspase 3
• Caspase 9

Topics

• Apoptosis (drug effects)
• Benzoquinones (isolation & purification, pharmacology)
• Caspase 3 (drug effects, metabolism)
• Caspase 9 (drug effects, metabolism)
• Cell Division (drug effects)
• Cell Survival (drug effects)
• DNA Fragmentation (drug effects)
• DNA, Neoplasm (drug effects)
• Humans
• Phenols (isolation & purification, pharmacology)
• Soil (analysis)
• U937 Cells (drug effects, pathology)