Is lysyl oxidase-like protein-1, alpha-1 antitrypsin, and neutrophil elastase site specific in pelvic organ prolapse?

Abstract

INTRODUCTION AND HYPOTHESIS:

We investigated whether the expression of alpha-1 antitrypsin (ATT), neutrophil elastase (NE), and lysyl oxidase-like protein 1 (LOXL-1) vary within the vagina in subjects with pelvic organ prolapse (POP).

METHODS:

Biopsies were obtained from the anterior and posterior vaginal wall of 22 women with POP (> or =stage 2 by POP-Q). The subjects were grouped by the most prominent defect: cystocele, cystocele plus uterine prolapse, and rectocele. Comparative real-time PCR, Western blotting, and NE enzyme activity assay were performed.

RESULTS:

The ratio of anterior and posterior vaginal wall ATT, NE, and LOXL-1 expression varied between individuals within the same defect group.

CONCLUSIONS:

ATT, NE, and LOXl-1 expression was variable among different biopsy sites in the vagina. No consistent pattern was present when the subjects were grouped by the most prominent defect. We recommend careful consideration of biopsy sites in future studies on POP to enhance reproducibility of data.

• Authors: Weng Chi Man, Jason Yen-Ping Ho, Yan Wen, Eric R Sokol, Mary L Polan, Bertha Chen
• Journal: International urogynecology journal and pelvic floor dysfunction (Int Urogynecol J Pelvic Floor Dysfunct) Vol. 20 Issue 12 Pg. 1423-9 (Dec 2009) England
• PMID: 19763368 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• alpha 1-Antitrypsin
• Amino Acid Oxidoreductases
• LOXL1 protein, human
• Leukocyte Elastase

Topics

• Adult
• Aged
• Aged, 80 and over
• Amino Acid Oxidoreductases (metabolism)
• Female
• Humans
• Leukocyte Elastase (metabolism)
• Middle Aged
• Pelvic Organ Prolapse (enzymology)
• Vagina (enzymology)
• alpha 1-Antitrypsin (metabolism)