Stimulatory effect of growth hormone-releasing hormone (GHRH(1-29)NH2) on the proliferation, VEGF and chromogranin A secretion by human neuroendocrine tumor cell line NCI-H727 in vitro.

Abstract

Growth hormone-releasing hormone (GHRH) and its receptors have been implicated in a variety of cellular processes like cell survival, proliferation, apoptosis, angiogenesis and neoplastic transformation of various non-pituitary tissues. Here, we investigated for the first time the in vitro effect of GHRH(1-29)NH2 on the proliferation and the secretion of vascular endothelial growth factor (VEGF) and chromogranin A by the human bronchial neuroendocrine tumor cells NCI-H727. GHRH(1-29)NH2 at the concentrations of 10(-8)-10(-6)M increased the proliferation of these cells and this effect was associated with a statistically significant increase in VEGF and chromogranin A secretion into the supernatants of the tested cells. Our findings indicate that GHRH functions as a trophic hormone for bronchial neuroendocrine (NET) tumors.

• Authors: Tomasz Stepień, Małgorzata Sacewicz, Hanna Lawnicka, Roman Krupiński, Jan Komorowski, Agnieszka Siejka, Henryk Stepień
• Journal: Neuropeptides (Neuropeptides) Vol. 43 Issue 5 Pg. 397-400 (Oct 2009) ISSN: 1532-2785 [Electronic] Netherlands
• PMID: 19747727 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Chromogranin A
• Vascular Endothelial Growth Factor A
• Sermorelin

Topics

• Animals
• Cell Line, Tumor (metabolism)
• Cell Proliferation (drug effects)
• Chromogranin A (metabolism)
• Humans
• Neuroendocrine Tumors (metabolism)
• Sermorelin (pharmacology)
• Vascular Endothelial Growth Factor A (metabolism)