Increased cerebral output of free radicals during hypoxia: implications for acute mountain sickness?

Abstract

This study examined whether hypoxia causes free radical-mediated disruption of the blood-brain barrier (BBB) and impaired cerebral oxidative metabolism and whether this has any bearing on neurological symptoms ascribed to acute mountain sickness (AMS). Ten men provided internal jugular vein and radial artery blood samples during normoxia and 9-h passive exposure to hypoxia (12.9% O(2)). Cerebral blood flow was determined by the Kety-Schmidt technique with net exchange calculated by the Fick principle. AMS and headache were determined with clinically validated questionnaires. Electron paramagnetic resonance spectroscopy and ozone-based chemiluminescence were employed for direct detection of spin-trapped free radicals and nitric oxide metabolites. Neuron-specific enolase (NSE), S100beta, and 3-nitrotyrosine (3-NT) were determined by ELISA. Hypoxia increased the arterio-jugular venous concentration difference (a-v(D)) and net cerebral output of lipid-derived alkoxyl-alkyl free radicals and lipid hydroperoxides (P < 0.05 vs. normoxia) that correlated with the increase in AMS/headache scores (r = -0.50 to -0.90, P < 0.05). This was associated with a reduction in a-v(D) and hence net cerebral uptake of plasma nitrite and increased cerebral output of 3-NT (P < 0.05 vs. normoxia) that also correlated against AMS/headache scores (r = 0.74-0.87, P < 0.05). In contrast, hypoxia did not alter the cerebral exchange of S100beta and both global cerebral oxidative metabolism (cerebral metabolic rate of oxygen) and neuronal integrity (NSE) were preserved (P > 0.05 vs. normoxia). These findings indicate that hypoxia stimulates cerebral oxidative-nitrative stress, which has broader implications for other clinical models of human disease characterized by hypoxemia. This may prove a risk factor for AMS by a mechanism that appears independent of impaired BBB function and cerebral oxidative metabolism.

• Authors: Damian M Bailey, Sarah Taudorf, Ronan M G Berg, Carsten Lundby, Jane McEneny, Ian S Young, Kevin A Evans, Philip E James, Angharad Shore, David A Hullin, Joe M McCord, Bente K Pedersen, Kirsten Möller
• Journal: American journal of physiology. Regulatory, integrative and comparative physiology (Am J Physiol Regul Integr Comp Physiol) Vol. 297 Issue 5 Pg. R1283-92 (Nov 2009) ISSN: 1522-1490 [Electronic] United States
• PMID: 19726713 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers
• Free Radicals
• Nerve Growth Factors
• S100 Calcium Binding Protein beta Subunit
• S100 Proteins
• 3-nitrotyrosine
• Tyrosine
• Phosphopyruvate Hydratase
• Oxygen

Topics

• Acute Disease
• Adult
• Altitude Sickness (epidemiology, metabolism, physiopathology)
• Biomarkers (metabolism)
• Blood-Brain Barrier (metabolism, physiopathology)
• Brain (blood supply, metabolism)
• Free Radicals (metabolism)
• Headache (metabolism, physiopathology)
• Health Surveys
• Humans
• Hypoxia (metabolism)
• Male
• Nerve Growth Factors (metabolism)
• Oxidative Stress (physiology)
• Oxygen (metabolism)
• Phosphopyruvate Hydratase (metabolism)
• Regional Blood Flow (physiology)
• Retrospective Studies
• Risk Factors
• S100 Calcium Binding Protein beta Subunit
• S100 Proteins (metabolism)
• Tyrosine (analogs & derivatives, metabolism)