Serum levels of matrix metalloproteinase-2 as a marker of intimal hyperplasia.

Abstract

BACKGROUND:

A primary component in the development of intimal hyperplasia (IH) in response to vascular injury is basement membrane remodeling. Matrix metalloproteinases (MMPs) play a major role in this process by degradation of basement membrane proteins, mainly collagen type IV. Vascular injury initiates an inflammatory cascade with the release of tumor necrosis factor-alpha (TNFalpha), interleukin-1beta (IL-1beta), and C-reactive protein (CRP). We hypothesize serum levels of these elements may serve as biomarkers of the development of IH.

METHODS AND RESULTS:

At baseline, 2, 7, 10, and 14 days post-balloon angioplasty of the carotid artery, rat tissue samples were stained with Masson trichrome elastin to examine IH. Intima:media ratios (I:M) increased significantly over time postinjury. Serum samples were collected at the time of tissue sampling, and levels of MMP-2, MMP-9, collagen type IV, TNFalpha, IL-1beta, and CRP were assayed using sandwich enzyme-linked immunosorbent assay (ELISA). MMP-2 serum levels at 7, 10, and 14 days postinjury were significantly elevated compared with baseline. Other elements were not significantly elevated.

CONCLUSION:

Early and persistent elevation in the serum levels of MMP-2 may be a useful biomarker of basement membrane remodeling and the presence of IH.

• Authors: A Mike Tummers, Deidra J H Mountain, J William Mix, Stacy S Kirkpatrick, David C Cassada, Scott L Stevens, Michael B Freeman, Mitchell H Goldman, Oscar H Grandas
• Journal: The Journal of surgical research (J Surg Res) Vol. 160 Issue 1 Pg. 9-13 (May 01 2010) ISSN: 1095-8673 [Electronic] United States
• PMID: 19726059 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2010 Elsevier Inc. All rights reserved.

Chemical References

• Biomarkers
• Collagen Type IV
• Cytokines
• Matrix Metalloproteinase 2

Topics

• Animals
• Biomarkers (blood)
• Carotid Artery Injuries (blood, pathology)
• Collagen Type IV (metabolism)
• Cytokines (blood)
• Female
• Hyperplasia
• Matrix Metalloproteinase 2 (blood)
• Postoperative Period
• Rats
• Rats, Sprague-Dawley
• Tunica Intima (pathology)