CCK2 receptor expression transforms non-tumorigenic human NCM356 colonic epithelial cells into tumor forming cells.

Abstract

Expression of gastrin and cholecystokinin 2 (CCK(2)) receptor splice variants (CCK(2)R and CCK(2i4sv)R) are upregulated in human colonic adenomas where they are thought to contribute to tumor growth and progression. To determine the effects of ectopic CCK(2) receptor variant expression on colonic epithelial cell growth in vitro and in vivo, we employed the non-tumorigenic colonic epithelial cell line, NCM356. Receptor expression was induced using a retroviral expression vector containing cDNAs for either CCK(2i4sv)R or CCK(2)R. RT-PCR and intracellular Ca(2+) ([Ca(2+)](i)) imaging of RIE/CCK(2)R cells treated with conditioned media (CM) from NCM356 revealed that NCM356 cells express gastrin mRNA and secrete endogenous, biologically active peptide. NCM356 cells expressing either CCK(2)R or CCK(2i4sv)R (71 and 81 fmol/mg, respectively) grew faster in vitro, and exhibited an increase in basal levels of phosphorylated ERK (pERK), compared with vector. CCK(2) receptor selective antagonist, YM022, partially inhibited the growth of both receptor-expressing NCM356 cells, but not the control cells. Inhibitors of mitogen activated protein kinase pathway (MEK/ERK) or protein kinase C (PKC) isozymes partially inhibited the elevated levels of basal pERK and in vitro growth of receptor-expressing cells. Vector-NCM356 cells did not form tumors in nude mice, whereas, either CCK(2) receptor-expressing cells formed large tumors. Autocrine activation CCK(2) receptor variants are sufficient to increase in vitro growth and tumorigenicity of non-transformed NCM356 colon epithelial cells through a pathway involving PKC and the MEK/ERK axis. These findings support the hypothesis that expression of gastrin and its receptors in human colonic adenomas contributes to tumor growth and progression.

• Authors: Celia Chao, Xueliang Han, Kirk Ives, Jeseong Park, Andrey A Kolokoltsov, Robert A Davey, Mary P Moyer, Mark R Hellmich
• Journal: International journal of cancer (Int J Cancer) Vol. 126 Issue 4 Pg. 864-75 (Feb 15 2010) ISSN: 1097-0215 [Electronic] United States
• PMID: 19697327 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• DNA Primers
• Gastrins
• Receptor, Cholecystokinin B
• Calcium

Topics

• Adenoma (pathology)
• Animals
• Calcium (metabolism)
• Carcinoma (genetics)
• Cell Culture Techniques (methods)
• Cell Division (genetics)
• Colon (metabolism, pathology, physiology)
• Colorectal Neoplasms (genetics, pathology)
• DNA Primers
• Disease Progression
• Gastrins (genetics, metabolism)
• Genetic Variation
• Humans
• Intestinal Mucosa (metabolism, pathology, physiology)
• Mice
• Mutation
• Neoplasm Staging
• Receptor, Cholecystokinin B (genetics)
• Reverse Transcriptase Polymerase Chain Reaction