Lentivirus-mediated shRNA interference targeting STAT3 inhibits human pancreatic cancer cell invasion.

Abstract

AIM:

To investigate RNA interference targeting signal transducer and activator of transcription-3 (STAT3) on invasion of human pancreatic cancer cells.

METHODS:

We constructed three plasmids of RNA interference targeting the STAT3 gene. After LV (lentivirus)-STAT3siRNA (STAT3 small interfering RNA) the vector was transfected into the human pancreatic cell line, SW1990 and cell proliferation was measured by the MTT assay. Flow cytometry was used to assess cell cycle. Vascular endothelial growth factor (VEGF) and matrix metalloproteinase-2 (MMP-2) mRNA and protein expression were examined by quantitative PCR and western blotting, respectively. The invasion ability of SW1990 cells was determined by cell invasion assay.

RESULTS:

We successfully constructed the LV-STAT3siRNA lentivirus vector and proved that it can suppress expression of STAT3 gene in SW1990 cells. RNA interference of STAT3 by the LV-STAT3siRNA construct significantly inhibited the growth of SW1990 cells, in addition to significantly decreasing both VEGF and MMP-2 mRNA and protein expression. Moreover, suppression of STAT3 by LV-STAT3siRNA decreased the invasion ability of SW1990 cells.

CONCLUSION:

The STAT3 signaling pathway may provide a novel therapeutic target for the treatment of pancreatic cancer since it inhibits the invasion ability of pancreatic cancer cells.

• Authors: Guang Yang, Chen Huang, Jun Cao, Ke-Jian Huang, Tao Jiang, Zheng-Jun Qiu
• Journal: World journal of gastroenterology (World J Gastroenterol) Vol. 15 Issue 30 Pg. 3757-66 (Aug 14 2009) ISSN: 2219-2840 [Electronic] United States
• PMID: 19673016 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• RNA, Small Interfering
• STAT3 Transcription Factor
• Matrix Metalloproteinase 2

Topics

• Cell Line, Tumor
• Cell Proliferation
• Genetic Vectors
• Humans
• Lentivirus (genetics, metabolism)
• Matrix Metalloproteinase 2 (genetics, metabolism)
• Neoplasm Invasiveness
• Pancreatic Neoplasms (genetics, metabolism, pathology, therapy)
• RNA Interference
• RNA, Small Interfering (genetics, metabolism)
• STAT3 Transcription Factor (genetics, metabolism)
• Signal Transduction (physiology)