Abstract | PURPOSE: METHODS: Two hundred thirty-three peripheral blood samples from 154 renal cell carcinoma patients were investigated for the presence of disseminated tumor cells by autoMACS technique and immunocytochemical staining of cytokeratin. The frequency of circulating tumor cells was analyzed statistically for correlation with relevant clinical data. RESULTS: Two kinds of tumor cells were detected: those with expression of cytokeratin 8/18 (CK+) and cells without a detectable cytokeratin expression, which we called large blue-stained cells with a tumorlike morphology. After following the CD45 autoMACS depletion protocol, we identified circulating tumor cells in 96 (41%) of 233 peripheral blood samples, which originated from 81 (53%) of 154 renal cell carcinoma patients. A significant correlation between the detection of circulating tumor cells and positive lymph node status (P < 0.001; chi(2) test) and the presence of synchronous metastases at the time of primary tumor resection (P = 0.014; chi(2) test) was found. In a multivariate Cox's regression hazard model, presence of CK+ circulating tumor cells was significantly correlated with poor overall survival for renal cell carcinoma patients (relative risk, 2.3; P = 0.048). CONCLUSIONS:
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Authors | Karen Bluemke, Udo Bilkenroth, Axel Meye, Susanne Fuessel, Christine Lautenschlaeger, Steffen Goebel, Andres Melchior, Hans Heynemann, Paolo Fornara, Helge Taubert |
Journal | Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology
(Cancer Epidemiol Biomarkers Prev)
Vol. 18
Issue 8
Pg. 2190-4
(Aug 2009)
ISSN: 1538-7755 [Electronic] United States |
PMID | 19661076
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers, Tumor
- Keratin-18
- Keratin-8
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Topics |
- Biomarkers, Tumor
(blood)
- Carcinoma, Renal Cell
(blood, mortality, pathology)
- Flow Cytometry
- Humans
- Immunohistochemistry
- Kaplan-Meier Estimate
- Keratin-18
(metabolism)
- Keratin-8
(metabolism)
- Kidney Neoplasms
(blood, mortality, pathology)
- Lymphatic Metastasis
(pathology)
- Neoplasm Metastasis
(pathology)
- Neoplastic Cells, Circulating
(metabolism, pathology)
- Prognosis
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