Abstract |
A screening study for TRAIL resistance-overcoming activity was carried out, and activity-guided fractionations of Thevetia peruviana and Sapindus emarginatus led to the isolation of four cardenolide glycosides (1-4) and four triterpenoid saponins (5-8), respectively. In particular, cardenolide glycosides (1 and 2) from T. peruviana were shown to have a significant reversal effect on TRAIL resistance in human gastric adenocarcinoma cells, and real-time PCR showed that thevefolin (2) enhanced mRNA expression of death receptor 4 (DR4) and DR5. In addition, 1H and 13C NMR characterizations are shown for thevefolin (2) for the first time.
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Authors | Takashi Miyagawa, Takashi Ohtsuki, Takashi Koyano, Thaworn Kowithayakorn, Masami Ishibashi |
Journal | Journal of natural products
(J Nat Prod)
Vol. 72
Issue 8
Pg. 1507-11
(Aug 2009)
ISSN: 1520-6025 [Electronic] United States |
PMID | 19594141
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cardenolides
- Receptors, TNF-Related Apoptosis-Inducing Ligand
- Saponins
- TNF-Related Apoptosis-Inducing Ligand
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Topics |
- Base Sequence
- Cardenolides
(chemistry, isolation & purification, pharmacology)
- Humans
- Molecular Structure
- Nuclear Magnetic Resonance, Biomolecular
- Plants, Medicinal
(chemistry)
- Receptors, TNF-Related Apoptosis-Inducing Ligand
(genetics)
- Sapindus
(chemistry)
- Saponins
(chemistry, isolation & purification, pharmacology)
- Stomach Neoplasms
(metabolism)
- TNF-Related Apoptosis-Inducing Ligand
(drug effects)
- Thailand
- Thevetia
(chemistry)
- Tumor Cells, Cultured
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