Fucoidan protects against dopaminergic neuron death in vivo and in vitro.

Abstract

Parkinson's disease is a neurodegenerative disorder of uncertain pathogenesis characterized by a loss of dopaminergic neurons in substantia nigra pars compacta, and can be modeled by the neurotoxin 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP). Oxidative stress may contribute to MPTP- and Parkinson's disease-related neurodegeneration. Fucoidan is a sulfated polysaccharide extracted from brown seaweeds which possesses a wide variety of biological activities including potent antioxidative effects. Here we investigated the effect of fucoidan treatment on locomoter activities of animals, striatal dopamine and its metabolites and survival of nigral dopaminergic neurons in MPTP-induced animal model of Parkinsonism in C57/BL mice in vivo and on the neuronal damage induced by 1-methyl-4-phenylpyridinium (MPP(+)) in vitro, and to study the possible mechanisms. When administered prior to MPTP, fucoidan reduced behavioral deficits, increased striatal dopamine and its metabolites levels, reduced cell death, and led to a marked increase in tyrosine hydroxylase expression relative to mice treated with MPTP alone. Furthermore, we found that fucoidan inhibited MPTP-induced lipid peroxidation and reduction of antioxidant enzyme activity. In addition, pre-treatment with fucoidan significantly protected against MPP(+)-induced damage in MN9D cells. Taken together, these findings suggest that fucoidan has protective effect in MPTP-induced neurotoxicity in this model of Parkinson's disease via its antioxidative activity.

• Authors: Dingzhen Luo, Quanbin Zhang, Haomin Wang, Yanqiu Cui, Zuoli Sun, Jian Yang, Yan Zheng, Jun Jia, Fen Yu, Xuan Wang, Xiaomin Wang
• Journal: European journal of pharmacology (Eur J Pharmacol) Vol. 617 Issue 1-3 Pg. 33-40 (Sep 01 2009) ISSN: 1879-0712 [Electronic] Netherlands
• PMID: 19545563 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antioxidants
• Neuroprotective Agents
• Polysaccharides
• 3,4-Dihydroxyphenylacetic Acid
• fucoidan
• Tyrosine 3-Monooxygenase
• 1-Methyl-4-phenylpyridinium
• Dopamine

Topics

• 1-Methyl-4-phenylpyridinium (toxicity)
• 3,4-Dihydroxyphenylacetic Acid (metabolism)
• Animals
• Antioxidants (pharmacology)
• Cell Death (drug effects)
• Cell Line
• Dopamine (deficiency, metabolism)
• Lipid Peroxidation (drug effects)
• MPTP Poisoning (pathology)
• Male
• Mice
• Mice, Inbred C57BL
• Motor Activity (drug effects)
• Neostriatum (drug effects, metabolism)
• Neurons (cytology, drug effects, enzymology, metabolism)
• Neuroprotective Agents (pharmacology)
• Polysaccharides (pharmacology)
• Tyrosine 3-Monooxygenase (metabolism)