Rapid detection of matrix metalloproteinase-7 and carcinoembryonic antigen mRNA expression in intraoperative pleural lavage samples using the reverse transcriptase loop-mediated isothermal amplification method.

Abstract

OBJECTIVE:

To detect the RNA of the tumor origin in intraoperative pleural lavage accurately by examining using rapid nucleic acid amplification method because patients with lung cancer often have recurrence detected by a cytologic examination of cancer cells in intraoperative pleural lavage.

STUDY DESIGN:

We used reverse transcriptase loop-mediated isothermal amplification (RT-LAMP), which enables rapid gene amplification, and examined the expression of matrix metalloproteinase-7 (MMP-7) and carcinoembryonic antigen (CEA) mRNA in intraoperative pleural lavage samples obtained from 79 consecutive surgical patients with lung adenocarcinoma.

RESULTS:

MMP-7 mRNA was detected in cancer tissues from 76 (96%) of those cases, while it was also detected in the intraoperative pleural lavage samples of 23 (30%) of those 76 cases. In addition, CEA mRNA was detected in cancer tissues from 55 (70%) cases and in 8 (15%) of the lavage samples. MMP-7 mRNA-positive cases showed a significantly positive correlation with the p and n factors, and moderate to poor differentiation. Three cases shown to be positive in cytology examinations were also positive for both MMP-7 and CEA mRNA by RT-LAMP.

CONCLUSION:

This detection method can be utilized during an operation and may provide useful information.

• Authors: Satoshi Ikeda, Masaharu Inagaki, Kazuhiko Takabe, Jiro Kumagai, Keiko Suzuki
• Journal: Acta cytologica (Acta Cytol) 2009 May-Jun Vol. 53 Issue 3 Pg. 283-91 ISSN: 0001-5547 [Print] Switzerland
• PMID: 19534268 (Publication Type: Journal Article)

Chemical References

• Biomarkers, Tumor
• Carcinoembryonic Antigen
• RNA, Messenger
• RNA, Neoplasm
• MMP7 protein, human
• Matrix Metalloproteinase 7

Topics

• Adenocarcinoma (diagnosis, genetics, surgery)
• Adult
• Aged
• Aged, 80 and over
• Biomarkers, Tumor (genetics, metabolism)
• Carcinoembryonic Antigen (genetics, metabolism)
• Cytodiagnosis (methods, trends)
• Female
• Gene Expression
• Humans
• Intraoperative Care
• Lung Neoplasms (diagnosis, genetics, surgery)
• Male
• Matrix Metalloproteinase 7 (genetics, metabolism)
• Middle Aged
• Pleural Cavity (pathology)
• RNA, Messenger (metabolism)
• RNA, Neoplasm (analysis)
• Reverse Transcriptase Polymerase Chain Reaction (methods)