Non-Hodgkin lymphoma (NHL) subtypes defined by common translocations: utility of fluorescence in situ hybridization (FISH) in a case-control study.

Abstract

We used fluorescence in situ hybridization (FISH) assays to identify t(14;18) translocations in archival paraffin-embedded tumor sections from non-Hodgkin lymphoma (NHL) cases enrolled in a population-based study. t(14;18) was identified in 54% of 152 cases, including 39% of diffuse large cell lymphomas (26 of 66 cases) and 84% of follicular lymphomas (36 of 43 cases). Eighty-seven percent of t(14;18)-positive cases and 57% of t(14;18)-negative cases expressed bcl-2. FISH assays detected twice as many t(14;18)-positive follicular lymphomas as PCR assays. Overall, study findings support the use of FISH assays to detect t(14;18) in archival tumor samples for epidemiologic studies of NHL subtypes.

• Authors: Cindy M Chang, Jane C Schroeder, Wen-Yi Huang, Cherie H Dunphy, Ralph S Baric, Andrew F Olshan, Kathleen C Dorsey, Georgette A Dent, James R Cerhan, Charles F Lynch, Nathaniel Rothman, Kenneth P Cantor, Aaron Blair
• Journal: Leukemia research (Leuk Res) Vol. 34 Issue 2 Pg. 190-5 (Feb 2010) ISSN: 1873-5835 [Electronic] England
• PMID: 19505720 (Publication Type: Evaluation Study, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2009 Elsevier Ltd. All rights reserved.

Chemical References

• Proto-Oncogene Proteins c-bcl-2

Topics

• Case-Control Studies
• Chromosomes, Human, Pair 14
• Chromosomes, Human, Pair 18
• Humans
• In Situ Hybridization, Fluorescence (methods, standards)
• Lymphoma, Follicular (genetics)
• Lymphoma, Large B-Cell, Diffuse (genetics)
• Lymphoma, Non-Hodgkin (classification, genetics)
• Proto-Oncogene Proteins c-bcl-2 (analysis)
• Translocation, Genetic