Abstract | ETHNOPHARMACOLOGICAL RELEVANCE: We characterized saponins as active constituents from traditionally used antidiabetic plant Helicteres isora. AIM OF THE STUDY: To evaluate the changes in the gene expression of the glucose and lipid metabolism regulating genes in C57BL/KsJ-db/db mice. MATERIALS AND METHODS: C57BL/KsJ-db/db mice were divided into four different groups; one diabetic control, the mice in other three groups were treated with methanol extract (100 mg/kg), saponins (100 mg/kg) and pioglitazone (30 mg/kg) for 14 days. After completion of the treatment period biochemical parameters and the expression levels of adipsin, adiponectin, glucose transporter 4 (Glut4), peroxisome proliferator activated receptor gamma ( PPARgamma), fatty acid binding protein 4 (FABP4), lipoprotein lipase (LPL) in adipose tissue and for liver RNA samples glucose-6-phosphatase (G6Pase), phosphoenolpyruvate carboxykinase (PEPCK), glucose transporter 2 (Glut2) and acyl-co- enzyme A oxidase (ACOX) were determined by quantitative real time PCR and angiopoeitin like 3 (ANGPTL3), angiopoeitin like 4 (ANGPTL4) and peroxisome proliferator activated receptor alpha ( PPARalpha) by semiquantitative reverse transcription PCR. RESULTS: Treatment caused a significant reduction in the serum lipid and glucose levels and increased the expression of adipsin, PPARgamma and Glut4 while reduced expression of FABP4 and G6Pase, whereas there was no effect on the expression levels of adiponectin, LPL, PEPCK, ACOX, Glut2, ANGPTL3, ANGPTL4 and PPARalpha. CONCLUSIONS:
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Authors | Shefalee K Bhavsar, Satinder Singh, Suresh Giri, Mukul R Jain, Dev D Santani |
Journal | Journal of ethnopharmacology
(J Ethnopharmacol)
Vol. 124
Issue 3
Pg. 426-33
(Jul 30 2009)
ISSN: 1872-7573 [Electronic] Ireland |
PMID | 19505560
(Publication Type: Journal Article)
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Chemical References |
- Angiopoietin-Like Protein 3
- Angiopoietin-Like Protein 4
- Angiopoietin-like Proteins
- Angiopoietins
- Angptl3 protein, mouse
- Angptl4 protein, mouse
- Blood Glucose
- Insulin
- PPAR alpha
- Plant Extracts
- RNA, Messenger
- Saponins
- Solvents
- Glucose
- Methanol
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Topics |
- Angiopoietin-Like Protein 3
- Angiopoietin-Like Protein 4
- Angiopoietin-like Proteins
- Angiopoietins
(biosynthesis, genetics)
- Animals
- Blood Glucose
(metabolism)
- Carbohydrate Metabolism
(drug effects, genetics)
- Diabetes Mellitus
(genetics)
- Gene Expression Regulation
(drug effects)
- Glucose
(metabolism)
- Glucose Tolerance Test
- Insulin
(blood)
- Lipid Metabolism
(drug effects, genetics)
- Liver
(drug effects, enzymology, metabolism)
- Malvaceae
(chemistry)
- Methanol
- Mice
- Mice, Inbred C57BL
- Mice, Obese
- PPAR alpha
(metabolism)
- Plant Extracts
(chemistry, pharmacology)
- Plant Roots
(chemistry)
- RNA, Messenger
(biosynthesis, isolation & purification)
- Saponins
(isolation & purification, pharmacology)
- Solvents
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