Induction of lipocalin-type prostaglandin D synthase in mouse heart under hypoxemia.

Abstract

Hypoxemia is a common manifestation of various disorders and generates pressure overload to the heart. Here we analyzed the expression of lipocalin-type prostaglandin D synthase (L-PGDS) in the heart of C57BL/6 mice kept under normobaric hypoxia (10% O2) that generates hemodynamic stress. Northern and Western blot analyses revealed that the expression levels of L-PGDS mRNA and protein were significantly increased (> twofold) after 14 days of hypoxia, compared to the mice kept under normoxia. Immunohistochemical analysis indicated that L-PGDS was increased in the myocardium of auricles and ventricles and the pulmonary venous myocardium at 28 days of hypoxia. Moreover, using C57BL/6 mice lacking heme oxygenase-2 (HO-2(-/-)), a model of chronic hypoxemia, we showed that the expression level of L-PGDS protein was twofold higher in the heart than that of wild-type mouse. L-PGDS expression is induced in the myocardium under hypoxemia, which may reflect the adaptation to the hemodynamic stress.

• Authors: Feng Han, Kazuhisa Takeda, Kazunobu Ishikawa, Masao Ono, Fumiko Date, Satoru Yokoyama, Kazumichi Furuyama, Yotaro Shinozawa, Yoshihiro Urade, Shigeki Shibahara
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 385 Issue 3 Pg. 449-53 (Jul 31 2009) ISSN: 1090-2104 [Electronic] United States
• PMID: 19470375 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Lipocalins
• Heme Oxygenase (Decyclizing)
• heme oxygenase-2
• Intramolecular Oxidoreductases
• prostaglandin R2 D-isomerase

Topics

• Animals
• Heme Oxygenase (Decyclizing) (genetics)
• Hypoxia (enzymology)
• Intramolecular Oxidoreductases (biosynthesis)
• Lipocalins (biosynthesis)
• Male
• Mice
• Mice, Inbred C57BL
• Mice, Mutant Strains
• Myocardium (enzymology)