Effects of antisense oligodeoxynucleotide to type I collagen gene on hypertrophic scars in the transplanted nude mouse model.

Abstract

BACKGROUND:

Antisense nucleic acids are effective in inhibiting harmful or uncontrolled gene expression. We had previously proved that the antisense DNA to type I collagen could effectively inhibit the synthesis of collagen type I in cultured hypertrophic scar fibroblasts, suggesting a potential role in anti-scarring, but there are no published reports of its effect on scar in the transplanted nude mouse model.

AIMS:

To investigate the effects of antisense oligodeoxynucleotide (ASODN) to type I collagen gene on hypertrophic scars in the transplanted nude mouse model and clarify the potential of ASODN for the treatment of scars.

METHODS:

The nudemousemodel of hypertrophic scar was created and subjected to daily injections with ASODN and LipofectamineTM for 2 ,4 or 6 weeks.We then examined the scars for changes in histopathological characteristics. The effects of ASODNon type I collagen gene expression were examined by reverse transcription-polymerase chain reaction and Western blots.

RESULTS:

The ASODN could remarkably alleviate the scar in the nude mouse model and consistently inhibit type I collagen gene expression at both the mRNA and protein levels.

CONCLUSION:

ASODN was effective in downregulating type I collagen gene expression and could prove to be useful in the treatment of scars.

• Authors: Julin Xie, Shaohai Qi, Jishan Yuan, Yinbing Xu, Tianzeng Li, Houdong Li, Xusheng Liu, Bin Shu, Huizeng Liang
• Journal: Journal of cutaneous pathology (J Cutan Pathol) Vol. 36 Issue 11 Pg. 1146-50 (Nov 2009) ISSN: 1600-0560 [Electronic] United States
• PMID: 19469869 (Publication Type: Journal Article)

Chemical References

• Collagen Type I
• Oligodeoxyribonucleotides, Antisense

Topics

• Animals
• Blotting, Western
• Cicatrix, Hypertrophic (genetics, prevention & control)
• Collagen Type I (genetics)
• Down-Regulation
• Enzyme-Linked Immunosorbent Assay
• Gene Expression (drug effects)
• Humans
• Mice
• Mice, Nude
• Oligodeoxyribonucleotides, Antisense (pharmacology)
• Reverse Transcriptase Polymerase Chain Reaction
• Skin Transplantation